Lipid composition of liver peroxisomes isolated from untreated and clofibrate-treated mice and rats

Comparative Biochemistry and Physiology Part B: Comparative Biochemistry Pub Date : 1994-12-01 DOI:10.1016/0305-0491(94)90129-5

Titus Kyrklund , Johan Meijer

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引用次数: 8

Abstract

Peroxisomes were isolated from liver tissue of control and clofibrate-treated adult male NMRI mice and Sprague-Dawley rats. Phospholipids, cholesterol, triglycerides and free fatty acids were measured in the peroxisomes. The fatty acid profiles of the phosphatidylethanolamine, the phosphatidylcholine, the triglyceride and the free fatty acid fractions were also analyzed. Phosphatidylethanolamine was the dominating phospholipid in peroxisomes from untreated animals. The fatty acid profiles of phosphatidylethanolamine, free fatty acids and triglycerides were similar for untreated mice and rats but differences between the species were observed in the pattern derived from phosphatidylcholine. Phosphatidylcholine was the most abundant phospholipid after clofibrate treatment. Clofibrate treatment caused an increase in the concentrations of phospholipids and unsaturated long-chain fatty acids and a decrease in the concentrations of triglycerides, free fatty acids, cholesterol and shorter saturated fatty acids.

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从未治疗和氯贝特治疗的小鼠和大鼠分离肝脏过氧化物酶体的脂质组成

从对照、氯贝特处理的成年雄性NMRI小鼠和Sprague-Dawley大鼠的肝组织中分离过氧化物酶体。测定过氧化物酶体中的磷脂、胆固醇、甘油三酯和游离脂肪酸。分析了磷脂酰乙醇胺、磷脂酰胆碱、甘油三酯和游离脂肪酸组分的脂肪酸谱。磷脂酰乙醇胺是未处理动物过氧化物酶体的主要磷脂。在未治疗的小鼠和大鼠中，磷脂酰乙醇胺、游离脂肪酸和甘油三酯的脂肪酸谱相似，但在磷脂酰胆碱衍生的模式中观察到不同物种之间的差异。氯贝特处理后，磷脂酰胆碱含量最高。氯贝特治疗引起磷脂和不饱和长链脂肪酸浓度的增加，甘油三酯、游离脂肪酸、胆固醇和短饱和脂肪酸浓度的降低。

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Comparative Biochemistry and Physiology Part B: Comparative Biochemistry

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