G. Sichel , C. Corsaro , E. Cassiani , C. Magnani , L. Bolognani
{"title":"ATPase activity of melanosomes in liver pigment cells of Rana esculenta L.","authors":"G. Sichel , C. Corsaro , E. Cassiani , C. Magnani , L. Bolognani","doi":"10.1016/0305-0491(94)90105-8","DOIUrl":null,"url":null,"abstract":"<div><p>Luminometric methods show that melanosomes in liver pigment cells of <em>Rana esculenta</em> L. have endogenous ATP and ATPase activity. The <em>K</em><sub>m</sub> value of ATPase is 0.42 × 10<sup>−8</sup> mol/l at pH 7.0. Inhibition of ATPase by antimycin and by ouabain is not effective. In the presence of an excess of ADP and Pi, ATP synthesis was observed.</p></div>","PeriodicalId":100294,"journal":{"name":"Comparative Biochemistry and Physiology Part B: Comparative Biochemistry","volume":"108 4","pages":"Pages 521-528"},"PeriodicalIF":0.0000,"publicationDate":"1994-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0305-0491(94)90105-8","citationCount":"1","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Comparative Biochemistry and Physiology Part B: Comparative Biochemistry","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/0305049194901058","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 1
Abstract
Luminometric methods show that melanosomes in liver pigment cells of Rana esculenta L. have endogenous ATP and ATPase activity. The Km value of ATPase is 0.42 × 10−8 mol/l at pH 7.0. Inhibition of ATPase by antimycin and by ouabain is not effective. In the presence of an excess of ADP and Pi, ATP synthesis was observed.