Use of human sera containing autoantibodies for an immunochemical study of some ribosomal proteins in rat, trout, mussel and fly maggot

Comparative Biochemistry and Physiology Part B: Comparative Biochemistry Pub Date : 1994-07-01 DOI:10.1016/0305-0491(94)90078-7

Jean-Claude Monier , Henri Perrier , Chantal Perrier , Agnés Desbos , Janine P. Bringuier

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引用次数: 1

Abstract

Sera from human subjects affected by autoimmune connective tissue diseases and containing antiribosomal autoantibodies were used to analyze by immunoblotting ribosomal proteins from trout (Oncorhynchus mykiss) liver, mussel (Mytilus edulis) hepatopancreas and whole fly maggots (Calliphora vomitoria). As usual in medical analysis of autoantibodies, the reference antigen preparation was extracted from rat liver. With the used sera, six known ribosomal proteins from rat liver were characterized: P₀, P₁, P₂, p30, p25 and p20. These six proteins were all targeted in trout; moreover an important 40 kDa fraction, undetectable in rat pattern, was seen. p30 and p20 were undetected in mussel and fly maggot; but p25, undetected in mussel, is clearly characterized in fly maggot. The interest of these data to infer phylogenic relationships is discussed.

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利用含有自身抗体的人血清对大鼠、鳟鱼、贻贝和蝇蛆的某些核糖体蛋白进行免疫化学研究

采用免疫印迹法对自身免疫性结缔组织疾病患者血清中含有抗核糖体自身抗体的鳟鱼(Oncorhynchus mykiss)肝脏、贻贝(Mytilus edulis)肝胰脏和全蝇蛆(Calliphora vomitoria)的核糖体蛋白进行分析。与医学分析自身抗体的方法一样，参考抗原制备方法是从大鼠肝脏中提取的。利用所使用的血清，鉴定了来自大鼠肝脏的六种已知核糖体蛋白:P0, P1, P2, p30, p25和p20。这六种蛋白都是鳟鱼的靶蛋白;此外，在大鼠模型中观察不到一个重要的40 kDa分数。贻贝和蝇蛆中未检出P30和p20;但在贻贝中未检测到的p25，在蝇蛆中明显存在。讨论了这些数据对推断系统发育关系的兴趣。

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Comparative Biochemistry and Physiology Part B: Comparative Biochemistry

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