cDNAs encoding for storage proteins in the tubers of taro (Colocasia esculenta Schott).

Idengaku zasshi Pub Date : 1993-06-01 DOI:10.1266/jjg.68.229
M Hirai, K Nakamura, T Imai, T Sato
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引用次数: 32

Abstract

Two major protein groups of taro (Colocasia esculenta) tuber were purified, and their antisera were used for the screening of the cDNA library constructed from poly(A)+ RNA of taro tuber. A cDNA clone obtained by screening with an anti-12 kD protein antiserum had an insert 1058 bp-long, and an open reading frame for a peptide of 268 amino acids. The analyses of the N-terminal amino acid sequence and in vitro translation product suggested that the protein was synthesized as a peptide with a molecular weight of 27 kD, and then processed into two mature peptides with a molecular weight of 12.5 and 13.9 kD and an extra peptide with a molecular weight of 0.6 kD. The cDNA clones obtained using the anti-25 kD protein antiserum were highly homologous with each other. One of them had an insert 958 bp-long and an open reading frame for a peptide with 209 amino acids. The amino acid sequence deduced from the nucleotide sequence of this clone indicated that the 25 kD proteins were homologous to the trypsin inhibitors of soybean and winged bean as well as sporamins, the storage proteins of sweet potato.

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芋头块茎中编码储存蛋白的cdna。
对芋头(Colocasia esculenta)块茎的两个主要蛋白群进行了纯化,并利用其抗血清筛选了由芋头聚(A)+ RNA构建的cDNA文库。用抗12kd蛋白抗血清筛选得到cDNA克隆,全长1058 bp,全长268个氨基酸的开放阅读框。n端氨基酸序列和体外翻译产物分析表明,该蛋白是作为一个分子量为27 kD的肽合成的,然后加工成两个分子量为12.5和13.9 kD的成熟肽和一个分子量为0.6 kD的额外肽。用抗25kd蛋白抗血清获得的cDNA克隆具有高度同源性。其中一个有一个958 bp长的插入和一个包含209个氨基酸的肽的开放阅读框。从该克隆的核苷酸序列推断,该25 kD蛋白与大豆、豇豆的胰蛋白酶抑制剂以及甘薯的贮藏蛋白孢菌素同源。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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