Mitosis of rotenone-induced endoreduplication in Chinese hamster cells.

Idengaku zasshi Pub Date : 1993-06-01 DOI:10.1266/jjg.68.185
K Matsumoto, T Ohta
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引用次数: 10

Abstract

Endoreduplication was induced by rotenone with an extremely high frequency (approximately 90% of all the metaphases) in cultured Chinese hamster cells. Endoreduplicated cells were fixed without colchicine or hypotonic treatment, and chromosomal configurations were examined in various mitotic stages. The two sister chromosomes of each diplochromosome at late prophase were widely separated except the centromeric region, but they became gradually paired along the total length as the cell cycle progressed to metaphase. The anaphase cells underwent multipolar division, resulting in three or four aneuploid daughter cells. Indirect immunofluorescence staining using anti-beta tubulin antibody revealed tripolar or tetrapolar spindles and unusual equatorial plates.

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鱼藤素诱导的中国仓鼠细胞内复制的有丝分裂。
鱼藤酮在培养的中国仓鼠细胞中以极高的频率(约占所有中期的90%)诱导细胞内重复。在没有秋水仙碱或低渗处理的情况下固定内复制细胞,并在有丝分裂的不同阶段检查染色体结构。在前期后期,除着丝粒区外,每条双倍染色体的两个姐妹染色体广泛分离,但随着细胞周期进入中期,它们沿全长逐渐配对。后期细胞发生多极分裂,产生3 ~ 4个非整倍体子细胞。抗微管抗体间接免疫荧光染色显示三极或四极纺锤体和不寻常的赤道板。
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