{"title":"[The sperm-agglutinating activity of the fractions of saline root extracts from Arum maculatum].","authors":"I Mladenov, I Bulanov, M Stamenova","doi":"","DOIUrl":null,"url":null,"abstract":"<p><p>Our previous investigations have demonstrated that saline extract of Arum maculatum contains lectin (sperm agglutinin) which agglutinates human ejaculated spermatozoa of the type tail to tail and head to tail. By means of speed liquid chromatography (Mono S XP 5/5) eight fractions (I-VIII) were obtained. The sperm-agglutinating activity of the fractions was investigated against frog testicular spermatozoa and testicular and epididymal spermatozoa of man, rabbit, rat and mouse. The fractions did not agglutinate frog spermatozoa. Fraction I agglutinated rabbit, rat and murine spermatozoa; fraction II-rabbit and rat spermatozoa; fraction VII-only human spermatozoa, and fraction VIII-human, as well as animal spermatozoa. The fractions III, IV, V, VI had no sperm-agglutinating activity. These data could be explained by the assumption that the differences in the sperm-agglutinating activity of the fractions are due to isoforms (called also isolectins) of the phytospermagglutinin, contained in the saline extract of Arum maculatum.</p>","PeriodicalId":11560,"journal":{"name":"Eksperimentalna meditsina i morfologiia","volume":"31 1-2","pages":"22-8"},"PeriodicalIF":0.0000,"publicationDate":"1993-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Eksperimentalna meditsina i morfologiia","FirstCategoryId":"1085","ListUrlMain":"","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0
Abstract
Our previous investigations have demonstrated that saline extract of Arum maculatum contains lectin (sperm agglutinin) which agglutinates human ejaculated spermatozoa of the type tail to tail and head to tail. By means of speed liquid chromatography (Mono S XP 5/5) eight fractions (I-VIII) were obtained. The sperm-agglutinating activity of the fractions was investigated against frog testicular spermatozoa and testicular and epididymal spermatozoa of man, rabbit, rat and mouse. The fractions did not agglutinate frog spermatozoa. Fraction I agglutinated rabbit, rat and murine spermatozoa; fraction II-rabbit and rat spermatozoa; fraction VII-only human spermatozoa, and fraction VIII-human, as well as animal spermatozoa. The fractions III, IV, V, VI had no sperm-agglutinating activity. These data could be explained by the assumption that the differences in the sperm-agglutinating activity of the fractions are due to isoforms (called also isolectins) of the phytospermagglutinin, contained in the saline extract of Arum maculatum.
我们以前的研究表明，盐提取物的Arum maculatum含有凝集素(精子凝集素)，凝集人类射精的精子类型尾尾和头尾。采用快速液相色谱法(Mono S XP 5/5)，得到8个组分(I-VIII)。研究了各组分对青蛙睾丸精子和人、兔、大鼠、小鼠睾丸及附睾精子的凝集活性。该组分不凝集青蛙精子。第一部分凝集兔、大鼠、鼠精子;部分ii -兔和大鼠精子;分数vii -仅人类精子，分数viii -人类和动物精子。部分III、IV、V、VI无凝集活性。这些数据可以通过这样的假设来解释，即不同组分的精子凝集活性的差异是由于植物精子凝集素的异构体(也称为隔离素)，这种异构体含有在Arum maculatum的盐水提取物中。
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