A novel mutation involved in the mitotic checkpoint in the fission yeast Schizosaccharomyces pombe.

Idengaku zasshi Pub Date : 1993-08-01 DOI:10.1266/jjg.68.265
J Ishiguro, N Yamada
{"title":"A novel mutation involved in the mitotic checkpoint in the fission yeast Schizosaccharomyces pombe.","authors":"J Ishiguro,&nbsp;N Yamada","doi":"10.1266/jjg.68.265","DOIUrl":null,"url":null,"abstract":"<p><p>The cps8 mutation which confers supersensitivity to a spindle poison, Isopropyl N-3-chlorophenyl carbamate (CIPC), in the fission yeast Schizosaccharomyces pombe was investigated. The cps8 mutant accumulated enlarged multinucleate cells in the stationary phase under normal growth conditions. The mutant was highly lethal at 36.5 degrees C in a fresh growth medium but not in a saline solution where the cell cycle ceases quickly. Lethality at high temperature was significantly suppressed by cdc1 or nda2 mutation which blocks nuclear division, but not by hydroxyurea treatment or cdc22 mutation which blocks DNA synthesis. A cdc10 cps8 double mutant remained lethal to high temperature, suggesting this double mutant to bypass the requirement for cdc10+ indispensable for the cell cycle start in a wild-type cell. After being transferred to a fresh medium at 36.5 degrees C, the multinucleate cells rapidly divided with aberrant nuclear segregation. Thus, cps8 mutation allows cells to undergo mitosis without DNA replication at the restrictive temperature. The cps8 gene was mapped on the left arm of chromosome II closely linked to but distinct from cdc2 locus.</p>","PeriodicalId":13120,"journal":{"name":"Idengaku zasshi","volume":"68 4","pages":"265-76"},"PeriodicalIF":0.0000,"publicationDate":"1993-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1266/jjg.68.265","citationCount":"7","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Idengaku zasshi","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1266/jjg.68.265","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 7

Abstract

The cps8 mutation which confers supersensitivity to a spindle poison, Isopropyl N-3-chlorophenyl carbamate (CIPC), in the fission yeast Schizosaccharomyces pombe was investigated. The cps8 mutant accumulated enlarged multinucleate cells in the stationary phase under normal growth conditions. The mutant was highly lethal at 36.5 degrees C in a fresh growth medium but not in a saline solution where the cell cycle ceases quickly. Lethality at high temperature was significantly suppressed by cdc1 or nda2 mutation which blocks nuclear division, but not by hydroxyurea treatment or cdc22 mutation which blocks DNA synthesis. A cdc10 cps8 double mutant remained lethal to high temperature, suggesting this double mutant to bypass the requirement for cdc10+ indispensable for the cell cycle start in a wild-type cell. After being transferred to a fresh medium at 36.5 degrees C, the multinucleate cells rapidly divided with aberrant nuclear segregation. Thus, cps8 mutation allows cells to undergo mitosis without DNA replication at the restrictive temperature. The cps8 gene was mapped on the left arm of chromosome II closely linked to but distinct from cdc2 locus.

查看原文
分享 分享
微信好友 朋友圈 QQ好友 复制链接
本刊更多论文
分裂酵母有丝分裂检查点的新突变。
研究了分裂酵母裂糖酵母(Schizosaccharomyces pombe)对纺锤体毒素异丙基n -3-氯苯氨基甲酸酯(Isopropyl N-3-chlorophenyl carbamate, CIPC)超敏感的cps8突变。在正常生长条件下,cps8突变体在固定期积累了增大的多核细胞。突变体在36.5℃的新鲜生长培养基中是高度致死的,但在细胞周期迅速停止的盐水溶液中不是。阻断核分裂的cdc1或nda2突变可显著抑制高温致死,而羟基脲处理或阻断DNA合成的cdc22突变则不能显著抑制高温致死。cdc10 - cps8双突变体在高温下仍然是致命的,这表明这种双突变体绕过了野生型细胞周期开始所必需的cdc10+。将多核细胞转移到36.5℃的新鲜培养基中后,多核细胞迅速分裂,细胞核分离异常。因此,cps8突变允许细胞在限制性温度下进行有丝分裂而不进行DNA复制。cps8基因被定位在II号染色体的左臂上,与cdc2位点有密切的联系,但与cdc2位点不同。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 去求助
来源期刊
自引率
0.00%
发文量
0
期刊最新文献
Mitochondrial plasmid-like DNAs of the B1 family in the genus Oryza: sequence heterogeneity and evolution. Molecular clock for dating of divergence between animal phyla. Regional localization of rat and mouse protein-tyrosine phosphatase PTP alpha/LRP gene (Ptpra) by fluorescence in situ hybridization. Polymorphic distribution and molecular diversification of mitochondrial plasmid-like DNAs in the genus Oryza. Adaptive significance of amylase polymorphism in Drosophila. X. Analysis of alpha-amylase activity of two amylase variants in individual Drosophila subobscura flies.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
0
微信
客服QQ
Book学术公众号 扫码关注我们
反馈
×
意见反馈
请填写您的意见或建议
请填写您的手机或邮箱
已复制链接
已复制链接
快去分享给好友吧!
我知道了
×
扫码分享
扫码分享
Book学术官方微信
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术
文献互助 智能选刊 最新文献 互助须知 联系我们:info@booksci.cn
Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。
Copyright © 2023 Book学术 All rights reserved.
ghs 京公网安备 11010802042870号 京ICP备2023020795号-1