Effect of insulin and glucagon on the mobility of ESR-probes incorporated in rat liver plasma membranes.

I Zlatanov, E Maltzeva, N Borovok, V Spassov
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引用次数: 3

Abstract

1. The effect of different concentrations of insulin (INS) and glucagon (GLU) on the rotational mobility of a membrane-incorporated spin probe 2,2-6,6-tetramethyl-4-capriloyl piperidine-1-oxil (C7) was investigated by electron spin resonance (ESR) technique. 2. Two strong adenylate cyclase effectors guanosine 5'-triphosphate (GTP) and guanylyl 5'-imidodiphosphate [Gpp(NH)p], as well as an antioxidant 4-methyl-2,6-ditretbutilphenol (AO) and a nonprotein hormone prostaglandin E2 (PGE2) were used as reference effectors. 3. Applied effectors reduced by 30-82% the rotation correlation time (TR) of the rat liver plasma membranes spin probe C7. The effect was time-dependent and reached saturation 30-40 min after the effector application. 4. The kinetic- and concentration-dependent changes in TR were described by a simple phenomenological model. The apparent binding constants and the number of the apparent membrane binding sites of the effectors used were calculated using the model.

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胰岛素和胰高血糖素对大鼠肝质膜esr探针移动性的影响。
1. 采用电子自旋共振(ESR)技术研究了不同浓度胰岛素(INS)和胰高血糖素(GLU)对膜结合自旋探针2,2-6,6-四甲基-4-capriloyl哌啶-1-oxil (C7)旋转迁移率的影响。2. 以两种强腺苷酸环化酶效应器鸟苷5′-三磷酸(GTP)和鸟酰5′-酰亚胺二磷酸[Gpp(NH)p],以及抗氧化剂4-甲基-2,6-二丁基酚(AO)和非蛋白激素前列腺素E2 (PGE2)作为参比效应器。3.应用效应器可使大鼠肝质膜自旋探针C7的旋转相关时间(TR)减少30 ~ 82%。效果是时间依赖性的,在效应器应用后30-40分钟达到饱和。4. 用一个简单的现象学模型描述了TR的动力学和浓度依赖性变化。利用该模型计算了所用效应器的表观结合常数和表观膜结合位点数。
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