[Comparison of several fluorescence HPLC methods for fumonisin analysis].

H Akiyama, M Miyahara, M Toyoda, Y Saito
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引用次数: 0

Abstract

Fumonisin has no particular cromophore groups in its chemical structure applicable for trace analysis, and therefore requires derivatization prior to measurement. Currently, the analysis of fumonisin B1 (FB1), B2 (FB2) is generally carried out after derivatizing FB1 mainly with o-phthalaldehyde to make a fluorescent compound (OPA-F). The OPA-F is, however, not sufficiently stable for precise analysis, and we have thus examined other fluorescent derivatives including fluorescamine, FMOC, NBD-F and DBD-F. The analytical method using FMOC as fluorescent agent required column chromatography after the derivatizing reaction, while reaction of FB1 with fluorescamine gave two chromatographic peaks. FMOC and fluorescamine are accordingly not desirable as fluorescent agents for analysis of small quantities of FB1 and FB2 in foods. In conclusion, for precise analysis of such trace amounts of FB1 and FB2, NBD-F or DBD-F should be used as fluorescent agents.

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几种荧光高效液相色谱法分析伏马菌素的比较
伏马菌素在其化学结构中没有特定的可用于痕量分析的色团,因此需要在测量前进行衍生化。目前对伏马菌素B1 (FB1)、B2 (FB2)的分析一般是在FB1主要与邻苯二醛衍生后进行,得到荧光化合物(OPA-F)。然而,OPA-F不够稳定,无法进行精确分析,因此我们研究了其他荧光衍生物,包括荧光胺、FMOC、NBD-F和DBD-F。以FMOC为荧光剂的分析方法在衍生化反应后需要进行柱层析,而FB1与荧光胺的反应有两个色谱峰。因此,FMOC和荧光胺不适合作为分析食品中少量FB1和FB2的荧光剂。综上所述,为了精确分析微量FB1和FB2,应该使用NBD-F或DBD-F作为荧光剂。
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