{"title":"Development of a diagnostic DNA probe to detect Setaria digitata: The causative parasite of cerebrospinal nematodiasis in goats, sheep and horses","authors":"W.S.S. Wijesundera, N.V. Chandrasekharan, E.H. Karunanayake, S.P. Dharmasena","doi":"10.1016/S0007-1935(96)80008-X","DOIUrl":null,"url":null,"abstract":"<div><p>Two repetitive sequences (IpSdM and IpSdS) have been cloned andsequenced from the genome of Setaria digitata. When IpSdM (214 bp) and IpSdS (201 bp) were aligned, a high degree of homology (85%) was observed, indicating that they belong to the same family of repeats. IpSdM represents a complete repeating element while IpSdS consists of two partial repeating elements arranged in tandem. The elements are present in about 10 000 copies comprising 2.8% of the <em>S. digilata</em> genome. As a diagnostic probe IpSdM detects as little as 100 pg DNA of both <em>S. digilata</em> and <em>S. labiato-papillosa</em>. It can also detect a single microfilaria and a L<sub>3</sub> larva making it a valuable tool to monitor cattle and mosquito vector populations in the prevention of cerebrospinal nematodiasis.</p></div>","PeriodicalId":100203,"journal":{"name":"British Veterinary Journal","volume":"152 5","pages":"Pages 561-571"},"PeriodicalIF":0.0000,"publicationDate":"1996-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0007-1935(96)80008-X","citationCount":"15","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"British Veterinary Journal","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S000719359680008X","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 15
Abstract
Two repetitive sequences (IpSdM and IpSdS) have been cloned andsequenced from the genome of Setaria digitata. When IpSdM (214 bp) and IpSdS (201 bp) were aligned, a high degree of homology (85%) was observed, indicating that they belong to the same family of repeats. IpSdM represents a complete repeating element while IpSdS consists of two partial repeating elements arranged in tandem. The elements are present in about 10 000 copies comprising 2.8% of the S. digilata genome. As a diagnostic probe IpSdM detects as little as 100 pg DNA of both S. digilata and S. labiato-papillosa. It can also detect a single microfilaria and a L3 larva making it a valuable tool to monitor cattle and mosquito vector populations in the prevention of cerebrospinal nematodiasis.
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