Diagnosis of rabbit haemorrhagic disease (RHD) and European brown hare syndrome (EBHS) by indirect sandwich polyclonal ELISA.

Zentralblatt fur Veterinarmedizin. Reihe B. Journal of veterinary medicine. Series B Pub Date : 1996-09-01 DOI:10.1111/j.1439-0450.1996.tb00331.x

S Nardelli, F Agnoletti, F Costantini, R Parpajola

引用次数: 5

Abstract

The establishment of two indirect sandwich polyclonal ELISAs for the virological diagnosis of rabbit haemorrhagic disease (RHD) and European brown hare syndrome (EBHS) is described. Each assay uses rabbit and guinea pig antisera raised to RHD or EBHS purified virus particles. The tests are sensitive and specific, but cross-reactions between rabbit haemorrhagic disease virus (RHDV) and European brown hare syndrome virus (EBHSV) are observed. Most rabbit liver homogenates identified as RHDV-positive by ELISA do not haemagglutinate human red blood cells at room temperature.

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间接夹心多克隆ELISA法诊断兔出血性疾病(RHD)和欧洲褐兔综合征(EBHS)。

本文建立了兔出血性病(RHD)和欧洲褐兔综合征(EBHS)病毒学诊断的间接夹心多克隆elisa。每次检测都使用兔和豚鼠抗血清进行RHD或EBHS纯化的病毒颗粒。该试验具有敏感性和特异性，但观察到兔出血性疾病病毒(RHDV)和欧洲褐兔综合征病毒(EBHSV)之间的交叉反应。大多数经ELISA鉴定为rhdv阳性的兔肝匀浆在室温下不能凝集人红细胞。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Zentralblatt fur Veterinarmedizin. Reihe B. Journal of veterinary medicine. Series B

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