[Purification of EcoO44I restriction endonuclease in Escherichia coli O44 isolated from an affected human].

M Miyahara, N Shinohara, K Mise
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Abstract

A restriction endonuclases (ENase) designated EcoO44I was purified without non specific nucleases from enteropathogenic Escherichia coli O44 Hiromi strain of affected human origin. The yield was 1, 100 units/g of wet cells. The EcoO44I ENase recognized and cleaved the specific sequence of 5'-GGTCTC-3' (1/5) as was the case with Eco31I or BsaI ENase. Because of the stability and high yield, EcoO44I would be useful for recombinant DNA technology after isolation of EcoO44-positive, avirulent mutant strains of E. coli O44 Hiromi.

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[人感染大肠杆菌O44中EcoO44I限制性内切酶的纯化]。
从人源肠致病性大肠埃希菌O44 Hiromi株中纯化了一种无特异性核酸酶的限制性内切酶EcoO44I。湿细胞产量为1100单位/g。与Eco31I或BsaI ENase一样,EcoO44I ENase识别并切割了5’-GGTCTC-3’(1/5)的特定序列。由于EcoO44I的稳定性和高产率,在分离出ecoo44阳性、无毒的大肠杆菌O44 Hiromi突变株后,EcoO44I可用于重组DNA技术。
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