[Change of calibration method for enzyme assay in clinical biochemistry using automatic analyzer--comparison of calibration methods using K factor and human standard serum].

M Saitoh, R Hasegawa, T Inoue
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Abstract

Enzyme activities in serum from experimental animals had been assayed by HITACHI 7150 Automatic Analyzer using K factors for calibration. Because K factor is derived from a molar extinction coefficient and, reagent and sample volumes for each assay system, it is a constant value in usual assay. As an alternative calibration method, a human standard serum, which is commercially available and well-controlled, is presently used in the same assay system because of some difficulties in supply. Four serum enzymes of human, rat, dog and monkey sera were determined by the above two methods. All values calibrated by human standard serum were approx. 10% higher than that using K factors. These small differences are allowable because data calibrated by human standard serum can be compared with previous data given by K factors.

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【临床生化全自动分析仪酶测定校准方法的改变——K因子与人标准血清校准方法的比较】。
用日立7150全自动酶分析仪测定实验动物血清酶活性,采用K因子进行校正。因为K因子是从摩尔消光系数和每个分析系统的试剂和样品体积中得出的,所以在通常的分析中它是一个恒定值。作为一种可供选择的校准方法,由于供应上的一些困难,目前在同一测定系统中使用的是市售的和控制良好的人标准血清。用上述两种方法测定了人、大鼠、狗和猴血清的四种血清酶。用人标准血清标定的所有值均接近。比使用K因子高出10%。这些微小的差异是允许的,因为通过人标准血清校准的数据可以与以前由K因子给出的数据进行比较。
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