[Sennosides Reference Standard (Control 951) of the National Institute of Health Sciences].

S Okada, A Kitajima, T Tanimoto, H Suzuki, M Satake
{"title":"[Sennosides Reference Standard (Control 951) of the National Institute of Health Sciences].","authors":"S Okada,&nbsp;A Kitajima,&nbsp;T Tanimoto,&nbsp;H Suzuki,&nbsp;M Satake","doi":"","DOIUrl":null,"url":null,"abstract":"<p><p>The \"Sennosides Reference Standard (Control 951)\" was prepared, which is intended to be used for the fluorophotometric assay of sennosides content in the preparation of \"Sennosides\". In this assay hydroxylated mono- and dianthraquinone glucosides are chelated with boric acid, and the fluorescence intensity of the chelate is determined against that of the Reference Standard (RS). In the establishment of this RS, sennosides content in the candidate material must be determined accurately by fluorophotometry. The Sennoside AB for assay, prepared as an equimolar mixture of the purified sennoside A and Sennoside B, was used as the RS for the fluorophotometry. Based on the above concept, sennosides content in the candidate was determined as calcium salts to be 60.1 +/- 1.6% by the fluorophotometry. Thus the sennosides content of this Sennosides RS was certified to be 60%. Separately, contents of Sennoside A (SA) and Sennoside B (SB) in this candidate were determined by using HPLC. As a result, the sum of SA and SB was estimated to be 38% as free acids. Thus it was suggested that about 20% of dianthraquinone glucosides other than SA and SB and anthraquinone glucosides may be included in this Sennoside RS as free acids. Analytical results on the USP Sennosides RS were also shown and discussed, compared with the present Sennosides RS.</p>","PeriodicalId":11656,"journal":{"name":"Eisei Shikenjo hokoku. Bulletin of National Institute of Hygienic Sciences","volume":" 114","pages":"106-12"},"PeriodicalIF":0.0000,"publicationDate":"1996-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Eisei Shikenjo hokoku. Bulletin of National Institute of Hygienic Sciences","FirstCategoryId":"1085","ListUrlMain":"","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0

Abstract

The "Sennosides Reference Standard (Control 951)" was prepared, which is intended to be used for the fluorophotometric assay of sennosides content in the preparation of "Sennosides". In this assay hydroxylated mono- and dianthraquinone glucosides are chelated with boric acid, and the fluorescence intensity of the chelate is determined against that of the Reference Standard (RS). In the establishment of this RS, sennosides content in the candidate material must be determined accurately by fluorophotometry. The Sennoside AB for assay, prepared as an equimolar mixture of the purified sennoside A and Sennoside B, was used as the RS for the fluorophotometry. Based on the above concept, sennosides content in the candidate was determined as calcium salts to be 60.1 +/- 1.6% by the fluorophotometry. Thus the sennosides content of this Sennosides RS was certified to be 60%. Separately, contents of Sennoside A (SA) and Sennoside B (SB) in this candidate were determined by using HPLC. As a result, the sum of SA and SB was estimated to be 38% as free acids. Thus it was suggested that about 20% of dianthraquinone glucosides other than SA and SB and anthraquinone glucosides may be included in this Sennoside RS as free acids. Analytical results on the USP Sennosides RS were also shown and discussed, compared with the present Sennosides RS.

分享 分享
微信好友 朋友圈 QQ好友 复制链接
本刊更多论文
[国家卫生科学研究所参比标准(Control 951)]。
制备了“番泻皂苷参比标准品(对照品951)”,用于“番泻皂苷”制剂中番泻皂苷含量的荧光光度测定。在本试验中,羟基化的单蒽醌和二蒽醌糖苷与硼酸螯合,并根据参考标准(RS)测定螯合物的荧光强度。在本标准体系的建立中,必须用荧光光度法准确测定候选物质中sen皂苷的含量。用纯化的Sennoside A和Sennoside B的等摩尔混合物制备用于测定的Sennoside AB作为荧光光度法的RS。基于上述概念,通过荧光光度法测定候选物中sen皂苷为钙盐的含量为60.1 +/- 1.6%。经鉴定,该RS中sennosides的含量为60%。分别用高效液相色谱法测定该候选物中Sennoside A (SA)和Sennoside B (SB)的含量。结果表明,SA和SB的游离酸之和约为38%。由此推测,除SA、SB和蒽醌类苷外,约20%的二蒽醌类苷可能以游离酸的形式存在于该Sennoside RS中。并对其分析结果进行了讨论,并与现行的参皂苷RS进行了比较。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 去求助
来源期刊
自引率
0.00%
发文量
0
期刊最新文献
[Cyanocobalamin Reference Standard (Control 951) of the National Institute of Health Sciences]. [dl-Camphor Reference Standard (Control 951) of the National Institute of Health Sciences]. [Lysozyme Reference Standard (Control 951) of the National Institute of Health Sciences]. [The Somatropin Reference Standard (Control 951) of the National Institute of Health Sciences]. [Studies on "Fast Green FCF Standard" for the dye standard on the National Institute of Health Sciences].
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
0
微信
客服QQ
Book学术公众号 扫码关注我们
反馈
×
意见反馈
请填写您的意见或建议
请填写您的手机或邮箱
已复制链接
已复制链接
快去分享给好友吧!
我知道了
×
扫码分享
扫码分享
Book学术官方微信
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术
文献互助 智能选刊 最新文献 互助须知 联系我们:info@booksci.cn
Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。
Copyright © 2023 Book学术 All rights reserved.
ghs 京公网安备 11010802042870号 京ICP备2023020795号-1