The contribution of newly synthesized cholesterol to biliary cholesterol in healthy humans.

J Scheibner, K Lange, K Empen, E F Stange
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引用次数: 1

Abstract

Hypersecretion of biliary cholesterol appears to be the key defect in the pathogenesis of cholesterol gallstones, and this may be due to an enhanced synthesis of cholesterol. To measure fractional syntheses of biliary and plasma cholesterol, five male and 3 female healthy humans with an intact enterohepatic circulation were infused intravenously with [1-13C]acetate for 15 h. Samples of duodenal bile and blood were taken hourly and an enteral formula diet was given. Free cholesterol mass distribution was analyzed by gas chromatography mass spectrometry. The Mass Isotopomer Distribution Analysis (MIDA) technique allowed to calculate fractional synthesis. After 6 hours of infusion, the [13C]label of the cytosolic acetate pool reached a plateau of approximately 12%. Individual fractional cholesterol synthesis is plasma and bile correlated significantly (6-15 h) and amounted to 4.2% and 5.3% after 15 h, respectively. It may be concluded from this study, that newly synthesized cholesterol is secreted into bile to a higher extent than into plasma.

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新合成胆固醇对健康人胆道胆固醇的贡献。
胆道胆固醇的高分泌似乎是胆固醇性胆结石发病机制中的关键缺陷,这可能是由于胆固醇合成增强所致。为了测量胆汁和血浆胆固醇的分数合成,将5名男性和3名女性肠肝循环完整的健康人静脉注射[1-13C]醋酸酯15小时。每小时采集一次十二指肠胆汁和血液样本,并给予肠内配方饮食。采用气相色谱-质谱法分析游离胆固醇的质量分布。质量同位素分布分析(MIDA)技术允许计算分数合成。注射6小时后,胞质乙酸池的[13C]标签达到约12%的平台。个体分数胆固醇合成血浆和胆汁显著相关(6-15 h), 15 h后分别达到4.2%和5.3%。从本研究可以得出结论,新合成的胆固醇分泌到胆汁中的程度高于分泌到血浆中的程度。
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