Immunohistochemical and biochemical analysis of laminin in neonatal rat first molars.

R Della Coletta, S S Veiga, S R Line
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引用次数: 3

Abstract

Rabbit polyclonal antibody against mouse EHS laminin was used to investigate the distribution and composition of laminin in the rat first molar tooth germ. Immunohistochemical analysis showed that laminin is expressed in the inner and outer epithelia of the enamel organ and in small blood vessels in the dental papilla and strellate reticulum. Immunoblots revealed that tooth germ laminin differs from EHS laminin. Tooth germ laminin contains beta chains, while the alpha 1 chain is substituted by a 300-kDa chain. Two-dimensional electrophoresis analysis of tooth germ extract showed that beta chains appeared as four spots with approximate pI values of 6.6, 7.5, 7.8 and 8.5. These results indicate that more than-one type of laminin isoform is present in the first molar tooth germ. Additionally, we have shown that despite the early degradation of tooth germ basement membrane, the laminin molecule is still intact at the time of birth.

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新生大鼠第一磨牙层粘连蛋白的免疫组织化学和生化分析。
采用兔抗小鼠EHS层粘连蛋白多克隆抗体,研究了层粘连蛋白在大鼠第一磨牙胚中的分布和组成。免疫组织化学分析表明,层粘连蛋白在牙釉质器官的内、外上皮以及牙乳头和星状网的小血管中表达。免疫印迹显示牙胚层粘连蛋白与EHS层粘连蛋白不同。牙胚层粘连蛋白含有β链,而α 1链被一条300 kda的链所取代。牙胚提取物的双向电泳分析显示,β链呈4个点状,pI值分别为6.6、7.5、7.8和8.5。这些结果表明,在第一磨牙胚芽中存在不止一种类型的层粘连蛋白异构体。此外,我们已经表明,尽管牙胚基膜的早期降解,层粘连蛋白分子在出生时仍然完整。
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A case of hemangiopericytoma of the buccal mucosa. A clinicopathologic study of odontomas: Malaysian findings. Immunohistochemical and biochemical analysis of laminin in neonatal rat first molars. An improved assay method for fibroblast gelatinolytic enzyme. Effects of unilateral upper incisor extraction on facial growth of young rats.
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