Antioxidant properties of butein isolated from Dalbergia odorifera

Zhi-Jiao Cheng , Sheng-Chu Kuo , Shiuh-Chuan Chan , Feng-Nien Ko , Che-Ming Teng
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引用次数: 127

Abstract

The antioxidant properties of butein, isolated from Dalbergia odorifera T. Chen, were investigated in this study. Butein inhibited iron-induced lipid peroxidation in rat brain homogenate in a concentration-dependent manner with an IC50, 3.3±0.4 μM. It was as potent as α-tocopherol in reducing the stable free radical diphenyl-2-picrylhydrazyl (DPPH) with an IC0.200, 9.2±1.8 μM. It also inhibited the activity of xanthine oxidase with an IC50, 5.9±0.3 μM. Besides, butein scavenged the peroxyl radical derived from 2,2-azobis(2-amidinopropane) dihydrochloride (AAPH) in aqueous phase, but not that from 2,2-azobis(2,4-dimethylvaleronitrile) (AMVN) in hexane. Furthermore, butein inhibited copper-catalyzed oxidation of human low-density lipoprotein (LDL), as measured by conjugated dienes and thiobarbituric acid-reactive substance (TBARS) formations, and electrophoretic mobility in a concentration-dependent manner. Spectral analysis revealed that butein was a chelator of ferrous and copper ions. It is proposed that butein serves as a powerful antioxidant against lipid and LDL peroxidation by its versatile free radical scavenging actions and metal ion chelation.

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降香黄檀中蛋白的抗氧化特性研究
研究了从降香黄檀中分离得到的蛋白的抗氧化性能。Butein抑制大鼠脑匀浆中铁诱导的脂质过氧化作用呈浓度依赖性,IC50为3.3±0.4 μM。对稳定自由基二苯基-2-picrylhydrazyl (DPPH)的还原作用与α-生育酚相当,ic0为0.200,9.2±1.8 μM。抑制黄嘌呤氧化酶活性的IC50值为5.9±0.3 μM。此外,丁蛋白在水溶液中清除了2,2-偶氮(2-氨基丙烷)二盐酸(AAPH)的过氧自由基,而在己烷中清除了2,2-偶氮(2,4-二甲基戊腈)(AMVN)的过氧自由基。此外,通过共轭二烯和硫代巴比妥酸反应物质(TBARS)的形成,丁蛋白抑制了铜催化的人低密度脂蛋白(LDL)氧化,并以浓度依赖的方式抑制了电泳迁移率。光谱分析表明,丁蛋白是铁离子和铜离子的螯合剂。研究表明,卵磷脂具有清除自由基和金属离子螯合作用,是一种有效的抗脂质和低密度脂蛋白过氧化的抗氧化剂。
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