Effects of electrolytic bilateral symmetric lesions of the arcuate nucleus on the phagocytic activity and on the phagocytic response in rats.

Abstract

Previous researches of Cluj-Napoca laboratories of Physiology (Benetato, Baciu et al., 1945, 1946, 1947) demonstrated that direct electrical stimulation of the tubero-mammillary area in dogs increases, in the following hours, the blood polymorphonuclears phagocytic activity. By contrast, electrical damage of the same region produces a depression of the basal phagocytic activity and a blocking of the phagocytic response (Baciu et al., 1958, 1988). In the present research we assumed there is a stimulating effect of the arcuate nucleus, located in this area, on the phagocytic activity of blood neutrophils. We used an anodal current to stereotaxically induce lesion of the arcuate nucleus in six rats. A control group of six animals was used. Five days later, phagocytic response was induced with a Gram negative bacterial extract given i.v. The results demonstrated a decrease of the phagocytic activity from 164.31 +/- 17 bacteria engulfed by 100 neutrophils in controls, to 138 +/- 12.8 in the lesioned group p < 0.05. Phagocytic response after five hours appears depressed in the lesioned group (138 +/- 12.8 to 156.25 +/- 13.3, p < 0.05). Similar results were obtained after 24 hours. In control animals the response is very significant after 5 and 24 hrs., respectively, (p < 0.001). In conclusion, the arcuate nucleus is moderately involved in sustaining the basal phagocytic activity of blood neutrophils. It has an important role in phagocytic response.