Flow cytometry: an overview.

B H Villas
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Abstract

Flow cytometry is an innovative technology that measures certain cell parameters as the cells flow in a fluid stream and in single file past an analytical laser light source. Clinical applications of flow cytometry currently utilized in the laboratory include cell surface antigen determinations or immunophenotyping of hematologic cells, DNA analysis of hematopoietic malignancies and solid tumors, and measurement of CD4 (T helper/inducer cell) absolute counts and T helper/T suppressor (CD4/CD8) ratios in the evaluation of immune deficiency. Flow cytometry often offers a more rapid, sensitive, accurate, and quantitative means of analyzing a particular cell population in a heterogeneous cell suspension as compared to more traditional microscopic methods. This article is intended to provide a general understanding of the technological basis of how a flow cytometer functions as well as an overview of both current and future flow cytometric clinical laboratory applications.

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流式细胞术:概述。
流式细胞术是一项创新技术,当细胞在流体中流动时,它可以测量细胞的某些参数,并在单个队列中通过分析激光光源。流式细胞术目前在实验室的临床应用包括血液细胞的细胞表面抗原测定或免疫表型分型,造血恶性肿瘤和实体瘤的DNA分析,以及免疫缺陷评价中CD4 (T辅助/诱导细胞)绝对计数和T辅助/T抑制(CD4/CD8)比值的测定。与传统的显微方法相比,流式细胞术通常提供了一种更快速、灵敏、准确和定量的方法来分析异质细胞悬液中的特定细胞群。本文旨在提供流式细胞仪功能的技术基础的一般理解以及当前和未来流式细胞仪临床实验室应用的概述。
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