首页 > 最新文献

Cell vision : the journal of analytical morphology最新文献

英文 中文
Technical considerations in immunocytochemistry. 免疫细胞化学的技术考虑。
J M Elias
{"title":"Technical considerations in immunocytochemistry.","authors":"J M Elias","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":79574,"journal":{"name":"Cell vision : the journal of analytical morphology","volume":"5 1","pages":"35-6"},"PeriodicalIF":0.0,"publicationDate":"1998-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"20578037","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
FISH: recent advances and diagnostic aspects. FISH:最近的进展和诊断方面。
S Luke, M Shepelsky

In situ Hybridization (ISH), since its introduction in 1969 by Gall and Pardue has found multiple uses in molecular morphology due to its unique capability of visualizing nucleic acid sequences without altering the cell's cytological, chromosomal or histological integrity. Fluorescence in situ Hybridization (FISH) has established itself as a variation of the traditional hybridization process whereby the probes utilized are a fluorescently labeled and produce bright clear signals upon detection. FISH is involved in localizing and exploring chromosomal, genetic and genomic aberrations that are often directly correlated to disease causation and progression. This technique is used as an essential tool not only in research laboratories, but also in prognostics, diagnostics and disease monitoring in medicine. In the past decade, there have appeared multiple modifications to traditional FISH technology allowing a wider variety of practical applications. Some of the latest innovative techniques discussed in this review paper are Forward and Reverse Chromosome Painting, Chromosome in situ suppression hybridization (CISS), Multicolor FISH, Chromosomal bar coding, Micro-FISH, In situ Hybridization to mRNA, in-cell RT-PCR, Fluorescence immunophonotyping and interphase cytogenetics as tool for investigation of neoplasmas (FICTION), Primed in situ DNA synthesis (PRINS), Fiber-FISH, FISHES, and Comparative genomic hybridization (CGH). This paper provides a brief overview of FISH and the techniques exploiting FISH modifications as well as their evaluation and discussion of practical applications.

原位杂交(ISH)自1969年由Gall和Pardue引入以来,由于其在不改变细胞细胞学,染色体或组织学完整性的情况下可视化核酸序列的独特能力,在分子形态学中发现了多种用途。荧光原位杂交(FISH)已经确立了自己作为传统杂交过程的一种变化,其中所利用的探针是荧光标记的,并在检测时产生明亮清晰的信号。FISH参与定位和探索染色体、遗传和基因组畸变,这些畸变通常与疾病的病因和进展直接相关。这项技术不仅在研究实验室中被用作基本工具,而且在医学上用于预后、诊断和疾病监测。在过去的十年中,对传统的FISH技术进行了多次修改,使其具有更广泛的实际应用。本文讨论了一些最新的创新技术,包括染色体正向和反向绘画,染色体原位抑制杂交(CISS),多色FISH,染色体条形码,Micro-FISH, mRNA原位杂交,细胞内RT-PCR,荧光免疫分型和间期细胞遗传学作为肿瘤研究工具(FICTION),引物原位DNA合成(PRINS),纤维-FISH, FISH和比较基因组杂交(CGH)。本文简要介绍了FISH和利用FISH修饰的技术,并对其实际应用进行了评价和讨论。
{"title":"FISH: recent advances and diagnostic aspects.","authors":"S Luke,&nbsp;M Shepelsky","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>In situ Hybridization (ISH), since its introduction in 1969 by Gall and Pardue has found multiple uses in molecular morphology due to its unique capability of visualizing nucleic acid sequences without altering the cell's cytological, chromosomal or histological integrity. Fluorescence in situ Hybridization (FISH) has established itself as a variation of the traditional hybridization process whereby the probes utilized are a fluorescently labeled and produce bright clear signals upon detection. FISH is involved in localizing and exploring chromosomal, genetic and genomic aberrations that are often directly correlated to disease causation and progression. This technique is used as an essential tool not only in research laboratories, but also in prognostics, diagnostics and disease monitoring in medicine. In the past decade, there have appeared multiple modifications to traditional FISH technology allowing a wider variety of practical applications. Some of the latest innovative techniques discussed in this review paper are Forward and Reverse Chromosome Painting, Chromosome in situ suppression hybridization (CISS), Multicolor FISH, Chromosomal bar coding, Micro-FISH, In situ Hybridization to mRNA, in-cell RT-PCR, Fluorescence immunophonotyping and interphase cytogenetics as tool for investigation of neoplasmas (FICTION), Primed in situ DNA synthesis (PRINS), Fiber-FISH, FISHES, and Comparative genomic hybridization (CGH). This paper provides a brief overview of FISH and the techniques exploiting FISH modifications as well as their evaluation and discussion of practical applications.</p>","PeriodicalId":79574,"journal":{"name":"Cell vision : the journal of analytical morphology","volume":"5 1","pages":"49-53"},"PeriodicalIF":0.0,"publicationDate":"1998-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"20578040","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Specific primer design and exonuclease III treatment for the reduction of nonspecific staining in direct in situ PCR. 在直接原位PCR中减少非特异性染色的特异性引物设计和外切酶III处理。
M Sagawa, J E Jones, Y Saito, S Fujimura, R I Linnoila

Although direct in situ PCR is more rapid than indirect method for the in situ identification of low copy number genes, several reports indicate serious nonspecific signals with this method. Some procedures have been reported in an effort to eliminate the nonspecific signals, but the results have not been satisfactory. Exonuclease III can progressively digest blunt or recessed 3' termini of double-stranded DNA whereas DNA with 3' overhanging end is resistant to digestion. DNA fragments amplified by PCR with primers incorporating the recognition site for Sph I generate 3' four bases extensions at both end after digestion. These fragments are expected to be resistant to exonuclease III digestion. It is also expected that nonspecifically incorporated digoxigenin would be released by treatment with exonuclease III thereby reducing background. We succeeded in digesting selectively with the samples after standard PCR by Sph I and exonuclease III treatment. However, we failed to eliminate the nonspecific signals of direct in situ PCR. Southern blotting revealed that the amount of nonspecific incorporation was so huge that exonuclease III was unable to release all of nonspecifically incorporated digoxigenin and maintain specific incorporated digoxigenin simultaneously. Direct in situ PCR is a sensitive method. However, its specificity has a significant problem.

虽然直接原位PCR比间接原位鉴定低拷贝数基因的方法更快,但一些报道表明这种方法存在严重的非特异性信号。已经报道了一些方法来消除非特异性信号,但结果并不令人满意。外切酶III可以逐步消化钝的或凹陷的双链DNA的3'末端,而具有3'悬垂末端的DNA则不易消化。用含有Sph I识别位点的引物扩增的DNA片段在酶切后在两端产生3' 4碱基延伸。预计这些片段能够抵抗外切酶III的酶切。也预期非特异性合并地高辛将通过外切酶III治疗释放,从而降低背景。经Sph I和外切酶III处理后,我们成功地对标准PCR后的样品进行选择性消化。然而,我们未能消除直接原位PCR的非特异性信号。Southern印迹显示,非特异性结合的数量如此之大,以至于外切酶III无法同时释放所有非特异性结合的地高辛并维持特异性结合的地高辛。直接原位PCR是一种灵敏的方法。然而,它的特异性存在显著的问题。
{"title":"Specific primer design and exonuclease III treatment for the reduction of nonspecific staining in direct in situ PCR.","authors":"M Sagawa,&nbsp;J E Jones,&nbsp;Y Saito,&nbsp;S Fujimura,&nbsp;R I Linnoila","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Although direct in situ PCR is more rapid than indirect method for the in situ identification of low copy number genes, several reports indicate serious nonspecific signals with this method. Some procedures have been reported in an effort to eliminate the nonspecific signals, but the results have not been satisfactory. Exonuclease III can progressively digest blunt or recessed 3' termini of double-stranded DNA whereas DNA with 3' overhanging end is resistant to digestion. DNA fragments amplified by PCR with primers incorporating the recognition site for Sph I generate 3' four bases extensions at both end after digestion. These fragments are expected to be resistant to exonuclease III digestion. It is also expected that nonspecifically incorporated digoxigenin would be released by treatment with exonuclease III thereby reducing background. We succeeded in digesting selectively with the samples after standard PCR by Sph I and exonuclease III treatment. However, we failed to eliminate the nonspecific signals of direct in situ PCR. Southern blotting revealed that the amount of nonspecific incorporation was so huge that exonuclease III was unable to release all of nonspecifically incorporated digoxigenin and maintain specific incorporated digoxigenin simultaneously. Direct in situ PCR is a sensitive method. However, its specificity has a significant problem.</p>","PeriodicalId":79574,"journal":{"name":"Cell vision : the journal of analytical morphology","volume":"5 1","pages":"20-3"},"PeriodicalIF":0.0,"publicationDate":"1998-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"20578843","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Molecular biology in transplantation medicine. 移植医学中的分子生物学。
R K Charlton

Serological methods of tissue typing have been used for matching patient-donor pairs for transplantation for several decades. Molecular techniques are rapidly being developed that can provide rapid, accurate tissue typing at the DNA sequence level. These techniques can be used to identify the best match pre-transplant and can also be used for the post-transplant monitoring of engraftment of bone marrow transplant patients.

几十年来,组织分型的血清学方法一直用于匹配移植的患者-供体配对。分子技术正在迅速发展,可以在DNA序列水平上提供快速、准确的组织分型。这些技术可用于确定移植前的最佳配型,也可用于骨髓移植患者移植后的移植监测。
{"title":"Molecular biology in transplantation medicine.","authors":"R K Charlton","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Serological methods of tissue typing have been used for matching patient-donor pairs for transplantation for several decades. Molecular techniques are rapidly being developed that can provide rapid, accurate tissue typing at the DNA sequence level. These techniques can be used to identify the best match pre-transplant and can also be used for the post-transplant monitoring of engraftment of bone marrow transplant patients.</p>","PeriodicalId":79574,"journal":{"name":"Cell vision : the journal of analytical morphology","volume":"5 1","pages":"54-5"},"PeriodicalIF":0.0,"publicationDate":"1998-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"20578041","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
The expanding role of molecular biology in clinical chemistry. 分子生物学在临床化学中的作用日益扩大。
R L Bertholf

Technical improvements in the application of molecular biology methods to detection and identification of specific nucleic acid sequences have resulted in more widespread incorporation of these techniques into clinical laboratory services. Gene amplification techniques have been refined and automated, making possible the rapid and economical detection of attomolar and smaller quantities of genetic material. Of particular benefit to clinical chemistry applications is the remarkable specificity of a DNA probe for its complementary sequence. Applications of molecular biology techniques in clinical chemistry include diagnosis of infectious, neoplastic, genetic, and hematological diseases. In addition, the use of oligonucleotides as molecular recognition probes may provide new analytical strategies for a wide range of diagnostic applications that currently rely on antibodies.

应用分子生物学方法检测和鉴定特定核酸序列的技术改进,导致这些技术更广泛地纳入临床实验室服务。基因扩增技术已经得到完善和自动化,使快速和经济地检测原子摩尔和少量遗传物质成为可能。对临床化学应用特别有益的是DNA探针对其互补序列的显著特异性。分子生物学技术在临床化学中的应用包括感染性、肿瘤性、遗传性和血液学疾病的诊断。此外,使用寡核苷酸作为分子识别探针可能为目前依赖抗体的广泛诊断应用提供新的分析策略。
{"title":"The expanding role of molecular biology in clinical chemistry.","authors":"R L Bertholf","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Technical improvements in the application of molecular biology methods to detection and identification of specific nucleic acid sequences have resulted in more widespread incorporation of these techniques into clinical laboratory services. Gene amplification techniques have been refined and automated, making possible the rapid and economical detection of attomolar and smaller quantities of genetic material. Of particular benefit to clinical chemistry applications is the remarkable specificity of a DNA probe for its complementary sequence. Applications of molecular biology techniques in clinical chemistry include diagnosis of infectious, neoplastic, genetic, and hematological diseases. In addition, the use of oligonucleotides as molecular recognition probes may provide new analytical strategies for a wide range of diagnostic applications that currently rely on antibodies.</p>","PeriodicalId":79574,"journal":{"name":"Cell vision : the journal of analytical morphology","volume":"5 1","pages":"67-9"},"PeriodicalIF":0.0,"publicationDate":"1998-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"20578044","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Morphological evidence for the location of calcitonin gene-related peptide (CGRP) immunoreactivity in rat lymphocytes. 大鼠淋巴细胞降钙素基因相关肽(CGRP)免疫反应性定位的形态学证据。
L Xing, J Guo, J Tang, Y Tang, X Wang

Calcitonin gene-related peptide (CGRP) is widely distributed in sensory neurons and nerve fibers. It was shown recently in our laboratory that there was CGRP-immunoreactivity (CGRP-ir) in extract of rat lymphocyte of thymus and mesenteric lymph node by radioimmunoassay and reversed-phase HPLC. The aim of this study was to detect the CGRP-ir location in the rat lymphocyte by immunocytochemical method. Single cells isolated from thymus and mesenteric lymph node of male Wistar rat (200-250 g) were suspended in RPMI-1640 medium. After adhesion to plate wall and through nylon wool fiber columns, T cell-riched suspension was obtained. Immunocytochemical ABC method was performed in cell suspension, the specific antiserum (1:200 diluted) was rabbit anti-human CGRP. The cells were examined under light microscope after smeared on glass slide by Shandon Cytospin. The results showed that some lymphocytes were CGRP-ir positive. The positive granules were seen as ring, plaque, cap or spot distributing on the surface or inside the cell. The results indicate that CGRP-ir may be located in some rat T lymphocytes. This work provides new information about the interaction between the nervous and the immune systems. The functional significance of CGRP located in lymphocytes needs to be further studied.

降钙素基因相关肽(CGRP)广泛分布于感觉神经元和神经纤维中。本实验室最近用放射免疫法和反相高效液相色谱法发现,大鼠胸腺和肠系膜淋巴结淋巴细胞提取物中存在cgrp免疫反应性(CGRP-ir)。本研究采用免疫细胞化学方法检测CGRP-ir在大鼠淋巴细胞中的位置。取雄性Wistar大鼠胸腺和肠系膜淋巴结单细胞(200-250 g),悬浮于RPMI-1640培养基中。附着于板壁后,通过尼龙羊毛纤维柱,得到富T细胞悬浮液。细胞悬液免疫细胞化学ABC法,特异性抗血清(1:20 00稀释)为兔抗人CGRP。用Shandon Cytospin涂片于载玻片上,光镜下观察。结果显示部分淋巴细胞CGRP-ir阳性。阳性颗粒呈环状、斑块、帽状或斑点状分布于细胞表面或细胞内部。提示CGRP-ir可能存在于部分大鼠T淋巴细胞中。这项工作为神经系统和免疫系统之间的相互作用提供了新的信息。位于淋巴细胞中的CGRP的功能意义有待进一步研究。
{"title":"Morphological evidence for the location of calcitonin gene-related peptide (CGRP) immunoreactivity in rat lymphocytes.","authors":"L Xing,&nbsp;J Guo,&nbsp;J Tang,&nbsp;Y Tang,&nbsp;X Wang","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Calcitonin gene-related peptide (CGRP) is widely distributed in sensory neurons and nerve fibers. It was shown recently in our laboratory that there was CGRP-immunoreactivity (CGRP-ir) in extract of rat lymphocyte of thymus and mesenteric lymph node by radioimmunoassay and reversed-phase HPLC. The aim of this study was to detect the CGRP-ir location in the rat lymphocyte by immunocytochemical method. Single cells isolated from thymus and mesenteric lymph node of male Wistar rat (200-250 g) were suspended in RPMI-1640 medium. After adhesion to plate wall and through nylon wool fiber columns, T cell-riched suspension was obtained. Immunocytochemical ABC method was performed in cell suspension, the specific antiserum (1:200 diluted) was rabbit anti-human CGRP. The cells were examined under light microscope after smeared on glass slide by Shandon Cytospin. The results showed that some lymphocytes were CGRP-ir positive. The positive granules were seen as ring, plaque, cap or spot distributing on the surface or inside the cell. The results indicate that CGRP-ir may be located in some rat T lymphocytes. This work provides new information about the interaction between the nervous and the immune systems. The functional significance of CGRP located in lymphocytes needs to be further studied.</p>","PeriodicalId":79574,"journal":{"name":"Cell vision : the journal of analytical morphology","volume":"5 1","pages":"8-12"},"PeriodicalIF":0.0,"publicationDate":"1998-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"20578841","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Optimization of fluorescence in situ hybridization (FISH) in cytological specimens. 细胞学标本荧光原位杂交(FISH)的优化。
S Luke, N F Huang, N Goyal, J A Varkey, A A Alsay-Oculam, M Gupta, C T Ladoulis
{"title":"Optimization of fluorescence in situ hybridization (FISH) in cytological specimens.","authors":"S Luke,&nbsp;N F Huang,&nbsp;N Goyal,&nbsp;J A Varkey,&nbsp;A A Alsay-Oculam,&nbsp;M Gupta,&nbsp;C T Ladoulis","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":79574,"journal":{"name":"Cell vision : the journal of analytical morphology","volume":"5 1","pages":"85-6"},"PeriodicalIF":0.0,"publicationDate":"1998-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"20577993","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Recent advances in telepathology. 心灵病理学的最新进展。
J Gu

Telepathology is to transform microscopic and gross images of pathological specimen into digital forms and transmit them electronically to remote sites for consultation and collaboration. Many benefits can be derived from telepathology. Although still at its initial developing stage, this technology is beginning to change the way pathological practice is conducted. With the rapid advancement of computer and telecommunication sciences, telepathology is increasingly becoming more feasible and practical for pathologists. This review gives a comprehensive account of the recent development in telepathology, discusses its functionality, applications, limitations and potential, and serves as an introduction to this rapidly evolving field.

远程病理学是将病理标本的显微和大体图像转换为数字形式,并以电子方式将其传输到远程站点以进行会诊和协作。心灵病理学可以带来许多好处。虽然这项技术还处于初级发展阶段,但它已经开始改变病理实践的方式。随着计算机和通信科学的飞速发展,对病理学家来说,心灵病理学越来越具有可行性和实用性。这篇综述全面介绍了心灵病理学的最新发展,讨论了它的功能、应用、局限性和潜力,并介绍了这个快速发展的领域。
{"title":"Recent advances in telepathology.","authors":"J Gu","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Telepathology is to transform microscopic and gross images of pathological specimen into digital forms and transmit them electronically to remote sites for consultation and collaboration. Many benefits can be derived from telepathology. Although still at its initial developing stage, this technology is beginning to change the way pathological practice is conducted. With the rapid advancement of computer and telecommunication sciences, telepathology is increasingly becoming more feasible and practical for pathologists. This review gives a comprehensive account of the recent development in telepathology, discusses its functionality, applications, limitations and potential, and serves as an introduction to this rapidly evolving field.</p>","PeriodicalId":79574,"journal":{"name":"Cell vision : the journal of analytical morphology","volume":"5 1","pages":"62-6"},"PeriodicalIF":0.0,"publicationDate":"1998-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"20578043","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
The economics of new trends in pathology. 病理学新趋势的经济学。
V M Anderson
{"title":"The economics of new trends in pathology.","authors":"V M Anderson","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":79574,"journal":{"name":"Cell vision : the journal of analytical morphology","volume":"5 1","pages":"71-2"},"PeriodicalIF":0.0,"publicationDate":"1998-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"20578046","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Detection of hepatitis C virus RNA in liver tissues by in situ hybridization. 原位杂交法检测肝组织中丙型肝炎病毒RNA。
C Xing, E Gong
{"title":"Detection of hepatitis C virus RNA in liver tissues by in situ hybridization.","authors":"C Xing,&nbsp;E Gong","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":79574,"journal":{"name":"Cell vision : the journal of analytical morphology","volume":"5 1","pages":"80-2"},"PeriodicalIF":0.0,"publicationDate":"1998-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"20577990","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
期刊
Cell vision : the journal of analytical morphology
全部 Acc. Chem. Res. ACS Applied Bio Materials ACS Appl. Electron. Mater. ACS Appl. Energy Mater. ACS Appl. Mater. Interfaces ACS Appl. Nano Mater. ACS Appl. Polym. Mater. ACS BIOMATER-SCI ENG ACS Catal. ACS Cent. Sci. ACS Chem. Biol. ACS Chemical Health & Safety ACS Chem. Neurosci. ACS Comb. Sci. ACS Earth Space Chem. ACS Energy Lett. ACS Infect. Dis. ACS Macro Lett. ACS Mater. Lett. ACS Med. Chem. Lett. ACS Nano ACS Omega ACS Photonics ACS Sens. ACS Sustainable Chem. Eng. ACS Synth. Biol. Anal. Chem. BIOCHEMISTRY-US Bioconjugate Chem. BIOMACROMOLECULES Chem. Res. Toxicol. Chem. Rev. Chem. Mater. CRYST GROWTH DES ENERG FUEL Environ. Sci. Technol. Environ. Sci. Technol. Lett. Eur. J. Inorg. Chem. IND ENG CHEM RES Inorg. Chem. J. Agric. Food. Chem. J. Chem. Eng. Data J. Chem. Educ. J. Chem. Inf. Model. J. Chem. Theory Comput. J. Med. Chem. J. Nat. Prod. J PROTEOME RES J. Am. Chem. Soc. LANGMUIR MACROMOLECULES Mol. Pharmaceutics Nano Lett. Org. Lett. ORG PROCESS RES DEV ORGANOMETALLICS J. Org. Chem. J. Phys. Chem. J. Phys. Chem. A J. Phys. Chem. B J. Phys. Chem. C J. Phys. Chem. Lett. Analyst Anal. Methods Biomater. Sci. Catal. Sci. Technol. Chem. Commun. Chem. Soc. Rev. CHEM EDUC RES PRACT CRYSTENGCOMM Dalton Trans. Energy Environ. Sci. ENVIRON SCI-NANO ENVIRON SCI-PROC IMP ENVIRON SCI-WAT RES Faraday Discuss. Food Funct. Green Chem. Inorg. Chem. Front. Integr. Biol. J. Anal. At. Spectrom. J. Mater. Chem. A J. Mater. Chem. B J. Mater. Chem. C Lab Chip Mater. Chem. Front. Mater. Horiz. MEDCHEMCOMM Metallomics Mol. Biosyst. Mol. Syst. Des. Eng. Nanoscale Nanoscale Horiz. Nat. Prod. Rep. New J. Chem. Org. Biomol. Chem. Org. Chem. Front. PHOTOCH PHOTOBIO SCI PCCP Polym. Chem.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
0
微信
客服QQ
Book学术公众号 扫码关注我们
反馈
×
意见反馈
请填写您的意见或建议
请填写您的手机或邮箱
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
Book学术官方微信
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术
文献互助 智能选刊 最新文献 互助须知 联系我们:info@booksci.cn
Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。
Copyright © 2023 Book学术 All rights reserved.
ghs 京公网安备 11010802042870号 京ICP备2023020795号-1