Catabolic fate of dietary trilinoleoylglycerol hydroperoxides in rat gastrointestines

Kazuki Kanazawa, Hitoshi Ashida
{"title":"Catabolic fate of dietary trilinoleoylglycerol hydroperoxides in rat gastrointestines","authors":"Kazuki Kanazawa,&nbsp;Hitoshi Ashida","doi":"10.1016/S0005-2760(98)00088-5","DOIUrl":null,"url":null,"abstract":"<div><p>To elucidate whether dietary lipid peroxides are absorbed in the body, the catabolic fate of trilinoleoylglycerol hydroperoxides (TL-OOH), in the gastrointestines of rats was examined. Oxidized trilinoleoylglycerol with a peroxide value of 1000 meq/kg, 0.5 or 20 mg, was dosed intragastrically to rat together with 59.5 or 40 mg unoxidized trilinoleoylglycerol, respectively. The fate of TL-OOH in gastric and intestinal lumina was determined by high-performance liquid chromatography periodically until 240 min after treatment. At low dose, TL-OOH was soon broken down to linoleic acid hydroperoxides (LA-OOH) and hydroxyls, probably through gastric lipases, whereas at high dose, TL-OOH was retained in the stomach. In both cases, TL-OOH did not reach the intestines, though the unoxidized lipids moved to the intestines. When LA-OOH was given intragastrically, the lipids decomposed in the stomach, and linoleic acid hydroxyls, hexanal, 9-oxononanoic acid, and two novel compounds were detected 30 min after treatment. The novel compounds were identified to be epoxyketones, 11-oxo-12,13-epoxy-9- and 11-oxo-9,10-epoxy-12-octadecenoic acids. Thus, dietary TL-OOH was broken down in the stomach releasing LA-OOH which decomposed further, and did not reach the intestines.</p></div>","PeriodicalId":100162,"journal":{"name":"Biochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"1998-08-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0005-2760(98)00088-5","citationCount":"47","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Biochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0005276098000885","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 47

Abstract

To elucidate whether dietary lipid peroxides are absorbed in the body, the catabolic fate of trilinoleoylglycerol hydroperoxides (TL-OOH), in the gastrointestines of rats was examined. Oxidized trilinoleoylglycerol with a peroxide value of 1000 meq/kg, 0.5 or 20 mg, was dosed intragastrically to rat together with 59.5 or 40 mg unoxidized trilinoleoylglycerol, respectively. The fate of TL-OOH in gastric and intestinal lumina was determined by high-performance liquid chromatography periodically until 240 min after treatment. At low dose, TL-OOH was soon broken down to linoleic acid hydroperoxides (LA-OOH) and hydroxyls, probably through gastric lipases, whereas at high dose, TL-OOH was retained in the stomach. In both cases, TL-OOH did not reach the intestines, though the unoxidized lipids moved to the intestines. When LA-OOH was given intragastrically, the lipids decomposed in the stomach, and linoleic acid hydroxyls, hexanal, 9-oxononanoic acid, and two novel compounds were detected 30 min after treatment. The novel compounds were identified to be epoxyketones, 11-oxo-12,13-epoxy-9- and 11-oxo-9,10-epoxy-12-octadecenoic acids. Thus, dietary TL-OOH was broken down in the stomach releasing LA-OOH which decomposed further, and did not reach the intestines.

查看原文
分享 分享
微信好友 朋友圈 QQ好友 复制链接
本刊更多论文
膳食中三叶油基甘油氢过氧化物在大鼠胃肠道中的分解代谢命运
为了阐明膳食脂质过氧化物是否在体内被吸收,研究了三叶油基甘油氢过氧化物(TL-OOH)在大鼠胃肠道中的分解代谢命运。过氧化值为1000 meq/kg、0.5或20 mg的氧化三叶油基甘油与59.5或40 mg未氧化三叶油基甘油分别灌胃给鼠。治疗后240 min,定期用高效液相色谱法测定胃、肠腔中TL-OOH的归宿。在低剂量下,TL-OOH可能通过胃脂肪酶分解为亚油酸氢过氧化物(LA-OOH)和羟基,而在高剂量下,TL-OOH在胃中保留。在这两种情况下,TL-OOH都没有到达肠道,尽管未氧化的脂质转移到了肠道。当LA-OOH灌胃时,脂质在胃中分解,并在治疗30 min后检测到亚油酸羟基、己醛、9-氧壬酸和两种新化合物。新化合物鉴定为环氧酮、11-氧-12,13-环氧-9和11-氧-9,10-环氧-12-十八烯酸。因此,膳食中的TL-OOH在胃中被分解,释放出LA-OOH, LA-OOH进一步分解,没有到达肠道。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 去求助
来源期刊
自引率
0.00%
发文量
0
期刊最新文献
Effect of methylglyoxal on the physico-chemical and biological properties of low-density lipoprotein Cholesterol synthesis is increased in mixed hyperlipidaemia The level of 7-dehydrocholesterol in plasma reflects the activity of 3-hydroxy-3-methylglutaryl coenzyme A reductase in the human liver Fatty acid α-oxidation of tetradecylthioacetic acid and tetradecylthiopropionic acid in cucumber (Cucumis sativus) Inductive electron-withdrawal from ammonium ion headgroups of cationic lipids and the influence on DNA transfection
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
0
微信
客服QQ
Book学术公众号 扫码关注我们
反馈
×
意见反馈
请填写您的意见或建议
请填写您的手机或邮箱
已复制链接
已复制链接
快去分享给好友吧!
我知道了
×
扫码分享
扫码分享
Book学术官方微信
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术
文献互助 智能选刊 最新文献 互助须知 联系我们:info@booksci.cn
Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。
Copyright © 2023 Book学术 All rights reserved.
ghs 京公网安备 11010802042870号 京ICP备2023020795号-1