Catabolic fate of dietary trilinoleoylglycerol hydroperoxides in rat gastrointestines

Kazuki Kanazawa, Hitoshi Ashida
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引用次数: 47

Abstract

To elucidate whether dietary lipid peroxides are absorbed in the body, the catabolic fate of trilinoleoylglycerol hydroperoxides (TL-OOH), in the gastrointestines of rats was examined. Oxidized trilinoleoylglycerol with a peroxide value of 1000 meq/kg, 0.5 or 20 mg, was dosed intragastrically to rat together with 59.5 or 40 mg unoxidized trilinoleoylglycerol, respectively. The fate of TL-OOH in gastric and intestinal lumina was determined by high-performance liquid chromatography periodically until 240 min after treatment. At low dose, TL-OOH was soon broken down to linoleic acid hydroperoxides (LA-OOH) and hydroxyls, probably through gastric lipases, whereas at high dose, TL-OOH was retained in the stomach. In both cases, TL-OOH did not reach the intestines, though the unoxidized lipids moved to the intestines. When LA-OOH was given intragastrically, the lipids decomposed in the stomach, and linoleic acid hydroxyls, hexanal, 9-oxononanoic acid, and two novel compounds were detected 30 min after treatment. The novel compounds were identified to be epoxyketones, 11-oxo-12,13-epoxy-9- and 11-oxo-9,10-epoxy-12-octadecenoic acids. Thus, dietary TL-OOH was broken down in the stomach releasing LA-OOH which decomposed further, and did not reach the intestines.

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膳食中三叶油基甘油氢过氧化物在大鼠胃肠道中的分解代谢命运
为了阐明膳食脂质过氧化物是否在体内被吸收,研究了三叶油基甘油氢过氧化物(TL-OOH)在大鼠胃肠道中的分解代谢命运。过氧化值为1000 meq/kg、0.5或20 mg的氧化三叶油基甘油与59.5或40 mg未氧化三叶油基甘油分别灌胃给鼠。治疗后240 min,定期用高效液相色谱法测定胃、肠腔中TL-OOH的归宿。在低剂量下,TL-OOH可能通过胃脂肪酶分解为亚油酸氢过氧化物(LA-OOH)和羟基,而在高剂量下,TL-OOH在胃中保留。在这两种情况下,TL-OOH都没有到达肠道,尽管未氧化的脂质转移到了肠道。当LA-OOH灌胃时,脂质在胃中分解,并在治疗30 min后检测到亚油酸羟基、己醛、9-氧壬酸和两种新化合物。新化合物鉴定为环氧酮、11-氧-12,13-环氧-9和11-氧-9,10-环氧-12-十八烯酸。因此,膳食中的TL-OOH在胃中被分解,释放出LA-OOH, LA-OOH进一步分解,没有到达肠道。
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