Distribution of 11-cis LRAT, 11-cis RD and 11-cis REH in bovine retinal pigment epithelium membranes

Biochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism Pub Date : 1998-10-02 DOI:10.1016/S0005-2760(98)00078-2

Nathan L. Mata, Andrew T.C. Tsin

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引用次数: 25

Abstract

Our recent finding of the co-localization of 11-cis retinyl esters and 11-cis retinyl ester hydrolase (11-cis REH) activity in bovine retinal pigment epithelium (RPE) plasma membrane (PM) has led us to explore the possibility that the PM may provide 11-cis retinal for rhodopsin regeneration. In the RPE, visual chromophore is synthesized via a membrane associated 11-cis retinol dehydrogenase (11-cis RD). Accordingly, bovine RPE membranes enriched with either endoplasmic reticulum (ER) or plasma membrane (PM) enzyme markers were prepared and assayed for visual cycle enzyme activities. Pronounced 11-cis RD activity was associated with both ER- and PM-enriched membrane fractions. In contrast, 11-cis REH activity was mostly recovered in PM-enriched fractions while LRAT activity was found only in ER-enriched membranes. The finding that both 11-cis retinol and 11-cis retinal can be produced at the PM of the bovine RPE strongly suggests that 11-cis retinyl esters at this subcellular locale serve as a precursor of visual chromophore for pigment regeneration.

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11-顺式LRAT、11-顺式RD和11-顺式REH在牛视网膜色素上皮膜中的分布

我们最近在牛视网膜色素上皮(RPE)质膜(PM)中发现了11-顺式视黄酰基酯和11-顺式视黄酰基酯水解酶(11-顺式REH)活性的共定位，这使我们探索了PM可能为视紫红质再生提供11-顺式视网膜的可能性。在RPE中，视觉发色团通过膜相关的11-顺式视黄醇脱氢酶(11-顺式RD)合成。因此，制备了含有内质网(ER)或质膜(PM)酶标记物的牛RPE膜，并测定了视觉循环酶活性。明显的11-顺式RD活性与ER和pm富集的膜组分有关。相比之下，11-顺式REH活性大部分在pm富集的部分恢复，而LRAT活性仅在er富集的膜中发现。发现11-顺式视黄醇和11-顺式视网膜都可以在牛RPE的PM产生，这强烈表明在这个亚细胞区域的11-顺式视黄醇酯作为色素再生的视觉发色团的前体。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Biochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism

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