Characterisation of triacylglycerol hydrolase activities in human placenta

Ian J. Waterman , Neil Emmison , Asim K. Dutta-Roy
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引用次数: 50

Abstract

Triacylglycerol hydrolase activities were characterised in homogenates, cytosol, and microvillous membranes (MVM) of human placenta. Homogenates of placenta exhibited three distinct triacylyglycerol hydrolase activities with pH optima 4.5, 6.0 and 8.0. On further fractionation, placental cytosol exhibited both acid cholesterol ester hydrolase (pH 4.5) and hormone sensitive lipase (pH 6.0) activities, whereas purified placental MVM exhibited two distinct triacylyglycerol hydrolase activities; a minor activity at pH 8.0 and a second major activity at pH 6.0. Triacylglycerol hydrolase activity at pH 8.0 of MVM appeared to be lipoprotein lipase (consistent with criteria such as serum stimulation and salt inhibition), whereas at pH 6.0 the activity was unique in that it was almost abolished by serum, but was not affected by high NaCl concentrations. Our data, for the first time, demonstrate that human placental MVM, in addition to lipoprotein lipase, contain a newly identified triacylglycerol hydrolase activity at pH 6.0.

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人胎盘三酰甘油水解酶活性的表征
在人胎盘匀浆、细胞质和微绒毛膜(MVM)中测定了三酰基甘油水解酶的活性。胎盘匀浆在pH为4.5、6.0和8.0时表现出三种不同的三酰基甘油水解酶活性。进一步分离后,胎盘细胞液显示出酸性胆固醇酯水解酶(pH为4.5)和激素敏感脂肪酶(pH为6.0)活性,而纯化后的胎盘MVM显示出两种不同的三酰甘油水解酶活性;pH值为8.0时次要活性,pH值为6.0时次要活性。MVM在pH 8.0时的三酰甘油水解酶活性似乎是脂蛋白脂肪酶(符合血清刺激和盐抑制等标准),而在pH 6.0时的活性则是独特的,它几乎被血清消除,但不受高NaCl浓度的影响。我们的数据首次证明,除了脂蛋白脂肪酶外,人胎盘MVM在pH 6.0时还含有一种新发现的三酰甘油水解酶活性。
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