[Biosynthesis of prostaglandins E2 and F2a by lung tissue in hypoxia and hyperthermia].

Abstract

Effect of a hypoxia and hyperthermia on endogenous biosynthesis of prostaglandins E2 and F2 alpha by tissues of lungs was studied. The effect of high temperature (43 degrees C-45 degrees C during I hour) and tissue hypoxemia by, exposition in an atmosphere with carbon monoxide, stimulated rise of formation of derivative of prostaglandins in the right and left lung: PGF2 alpha in 1.2-2.6, and PG E2-in 1.48-2.7 times accordingly. The parity between prostaglandins after experiment remained same, as well as in control group. The increase of a level of biosynthesis of investigated prostaglandins had more expressed character in a right lung, than in left. The reasons, leading to such significant growth of amounts of prostaglandins can be a few. One of them--it, apparently, growth of an amount of substrates for a phospholipase A2 and cyclooxygenase, as to for want of given temperature of a body is sharply increased a state of fluidity of membranes, leading to violation stability of membrane of phospholipids and further their destruction. Second--possible increase of activity of enzymes, leading to derivation of eicosanoids-cyclooxygenase and (or) of the phospholipase A2, leading to mobilization of an arachidonic acid from phospholipids of membranes. In a tissue of lungs after the hyperthermia and hypoxemia the decrease of amount of c-AMP on a background of c-GMP amount growth in the right and in the left lung was observed. For want of than, described above legitimacy was saved, in which the amount c-AMP in a right lung prevails over left. During realization of experiment interesting legitimacy-process, leading to growth the contents PG E2 was detected, also conducted to decrease of an amount c-AMP. The reduction of an amount c-AMP is possible, apparently, to explain the cell destruction, as heating in these parameters stimulates the cell apoptosis development, which conducts to reduction of an amount c-AMP inside a cell.