Genomic diversity of the genus Stenotrophomonas.

L Hauben, L Vauterin, E R Moore, B Hoste, J Swings
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引用次数: 157

Abstract

The clinical and environmental importance of Stenotrophomonas bacteria requires thorough, molecular studies on their epidemiology and taxonomy. In order to obtain a complete genomic profile of this genus, over 100 Stenotrophomonas maltophilia strains from various origins were investigated by AFLP fingerprinting. A subset of these strains was analysed by DNA hybridization and 16S rDNA sequencing. In contrast to their high phenotypic homogeneity, the strains were found to be very heterogeneous genotypically by AFLP fingerprinting. Nevertheless, ten cores of highly similar strains representing ten genomic groups were observed. The same groups could be retrieved by DNA hybridizations and also, partly, by 16S rDNA sequence analysis. The intergroup DNA similarities were too high to create confident species delineations, neither could the genomic groups be characterized by phenotypic features.

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窄食单胞菌属的基因组多样性。
窄养单胞菌的临床和环境重要性需要对其流行病学和分类进行彻底的分子研究。为了获得该属的完整基因组图谱,对来自不同来源的100多株嗜麦芽窄养单胞菌进行了AFLP指纹图谱研究。通过DNA杂交和16S rDNA测序对这些菌株进行了分析。与它们的高表型同质性相反,AFLP指纹图谱发现菌株的基因特征非常异质性。然而,十个核心高度相似的菌株代表十个基因组组被观察到。通过DNA杂交和部分16S rDNA序列分析,可以检索到相同的群体。组间DNA相似性太高,无法建立自信的物种描述,也无法通过表型特征来表征基因组组。
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