Dynamics of experimental vasogenic brain oedema in the rat: changes induced by adrenergic drugs

N. Borges, A. Sarmento, I. Azevedo
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引用次数: 13

Abstract

1 The effects of adrenergic drugs on the formation and resolution of cerebral oedema in a rat model of cold-induced vasogenic brain oedema were studied. Evans blue dye extravasation, water content and ultrastructural alterations (pinocytotic vesicle formation in capillary endothelial cells and apparent water accumulation in the brain parenchyma) were evaluated in parietal cortex.

2 Previous administration of the α-adrenoceptor antagonist phenoxybenzamine produced a reduction of Evans blue extravasation and water content, diminished vesicle formation and reduced water accumulation. Previous administration of the β2-adrenoceptor agonist clenbuterol reduced Evans blue extravasation and water content, but did not change vesicle frequency.

3 The effects of clenbuterol on Evans blue passage to the brain were blocked by timolol (β-adrenoceptor antagonist) but not by metoprolol (selective β1-adrenoceptor antagonist). When given after the application of cold, clenbuterol was also able to reduce Evans blue and water content in the brain. Isoprenaline (β-adrenoceptor agonist that does not cross the blood–brain barrier) showed a reduction in Evans blue extravasation only when given intracerebroventricularly. Vinblastine (a drug that prevents vesicle formation) produced a reduction of the amount of pinocytotic vesicles.

4 We conclude that there is an influence of the central adrenergic nervous system on the formation and/or resolution of vasogenic brain oedema and that the alterations on water movement and Evans blue transport mediated by adrenergic drugs seem to be due, at least in part, to alterations of pinocytotic activity in capillary endothelial cells.

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实验性大鼠血管源性脑水肿动力学:肾上腺素能药物引起的变化
研究肾上腺素能药物对大鼠冷致血管源性脑水肿形成及消退的影响。在顶叶皮层观察Evans蓝染料外渗、水含量和超微结构改变(毛细血管内皮细胞胞浆性囊泡形成和脑实质明显水积聚)。2先前给予α-肾上腺素能受体拮抗剂苯氧苄胺可减少埃文斯蓝外渗和水分含量,减少囊泡形成和减少水分积聚。先前给予β2-肾上腺素能受体激动剂克仑特罗可减少埃文斯蓝外渗和水分含量,但未改变囊泡频率。盐酸噻洛尔(β-肾上腺素能受体拮抗剂)可阻断盐酸克仑特罗对Evans蓝通道通往大脑的影响,而美托洛尔(选择性β-肾上腺素能受体拮抗剂)则不能。在冷敷后给药,克伦特罗也能减少大脑中的埃文斯蓝和水分含量。异丙肾上腺素(不穿过血脑屏障的β-肾上腺素受体激动剂)仅在脑室内给予时显示埃文斯蓝外渗的减少。长春碱(一种防止囊泡形成的药物)可以减少胞泡的数量。我们得出结论,中枢肾上腺素能神经系统对血管源性脑水肿的形成和/或消退有影响,肾上腺素能药物介导的水运动和埃文斯蓝转运的改变似乎至少部分是由于毛细血管内皮细胞的胞浆活性的改变。
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Issue Information Autonomic and Autacoid Pharmacology: Goodbye and thank you Attenuation of the anti-contractile effect of cooling in the rat aorta by perivascular adipose tissue Retraction: Dopamine receptor immunohistochemistry in the rat choroid plexus. Issue Information
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