Attenuation of the anti-contractile effect of cooling in the rat aorta by perivascular adipose tissue

Y. Rafique, M. AlBader, M. Oriowo
{"title":"Attenuation of the anti-contractile effect of cooling in the rat aorta by perivascular adipose tissue","authors":"Y. Rafique,&nbsp;M. AlBader,&nbsp;M. Oriowo","doi":"10.1111/aap.12058","DOIUrl":null,"url":null,"abstract":"<div>\n \n <p>\n \n </p><ol>\n \n \n <li>In addition to providing mechanical support for blood vessels, the perivascular adipose tissue (PVAT) secretes a number of vasoactive substances and exerts an anticontractile effect. The main objective of this study was to find out whether the anticontractile effect of cooling in the rat aorta is affected by PVAT. Our hypothesis was that PVAT would enhance the anticontractile effect of cooling in the rat aorta.</li>\n \n \n <li>Aorta segments, with or without PVAT, were used in this investigation. Cumulative concentration-response curves were established for phenylephrine at 37°C or 24°C. Phenylephrine (10<sup>-9</sup>M – 10<sup>-5</sup>M) induced concentration-dependent contractions of aorta segments with or without PVAT at 37°C. The maximum response, but not pD<sub>2</sub> value, was reduced in aorta segments with PVAT.</li>\n \n \n <li>Cooling the tissues to 24 °C resulted in a significant reduction in the maximum response in aorta segments without PVAT with no change in pD<sub>2</sub> values. However, the anticontractile effect of cooling was attenuated in the presence of PVAT with no significant (p &gt; 0.05) change in either the maximum response or pD<sub>2</sub> value.</li>\n \n \n <li>L-NAME potentiated PE-induced contractions and this was greater in aorta segments without PVAT at both temperatures.</li>\n \n \n <li>The expression of eNOS protein and basal tissue level of nitric oxide (NO) were greater in aorta segments with PVAT at both temperatures. However, PE significantly increased tissue levels of NO only in aorta segments without PVAT.</li>\n \n \n <li>We concluded that PVAT-induced loss of anticontractile effect of cooling against PE-induced contractions could be due to impaired generation of NO in aorta segments with PVAT.</li>\n </ol>\n \n </div>","PeriodicalId":100151,"journal":{"name":"Autonomic and Autacoid Pharmacology","volume":"37 4","pages":"52-60"},"PeriodicalIF":0.0000,"publicationDate":"2017-09-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1111/aap.12058","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Autonomic and Autacoid Pharmacology","FirstCategoryId":"1085","ListUrlMain":"https://onlinelibrary.wiley.com/doi/10.1111/aap.12058","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0

Abstract

  1. In addition to providing mechanical support for blood vessels, the perivascular adipose tissue (PVAT) secretes a number of vasoactive substances and exerts an anticontractile effect. The main objective of this study was to find out whether the anticontractile effect of cooling in the rat aorta is affected by PVAT. Our hypothesis was that PVAT would enhance the anticontractile effect of cooling in the rat aorta.
  2. Aorta segments, with or without PVAT, were used in this investigation. Cumulative concentration-response curves were established for phenylephrine at 37°C or 24°C. Phenylephrine (10-9M – 10-5M) induced concentration-dependent contractions of aorta segments with or without PVAT at 37°C. The maximum response, but not pD2 value, was reduced in aorta segments with PVAT.
  3. Cooling the tissues to 24 °C resulted in a significant reduction in the maximum response in aorta segments without PVAT with no change in pD2 values. However, the anticontractile effect of cooling was attenuated in the presence of PVAT with no significant (p > 0.05) change in either the maximum response or pD2 value.
  4. L-NAME potentiated PE-induced contractions and this was greater in aorta segments without PVAT at both temperatures.
  5. The expression of eNOS protein and basal tissue level of nitric oxide (NO) were greater in aorta segments with PVAT at both temperatures. However, PE significantly increased tissue levels of NO only in aorta segments without PVAT.
  6. We concluded that PVAT-induced loss of anticontractile effect of cooling against PE-induced contractions could be due to impaired generation of NO in aorta segments with PVAT.
查看原文
分享 分享
微信好友 朋友圈 QQ好友 复制链接
本刊更多论文
血管周围脂肪组织对大鼠主动脉降温抗收缩作用的衰减
除了为血管提供机械支持外,血管周围脂肪组织(PVAT)还分泌许多血管活性物质并发挥抗收缩作用。本研究的主要目的是探讨PVAT是否影响大鼠主动脉降温的抗收缩作用。我们的假设是PVAT会增强大鼠主动脉冷却的抗收缩作用。主动脉段,有无PVAT,用于本研究。建立苯肾上腺素在37°C和24°C时的累积浓度-响应曲线。在37℃时,苯肾上腺素(10-9M - 10-5M)诱导有或无PVAT的主动脉段的浓度依赖性收缩。主动脉段PVAT的最大反应降低,但不降低pD2值。将组织冷却至24°C导致无PVAT的主动脉段最大反应显著降低,而pD2值没有变化。然而,在PVAT的存在下,冷却的抗收缩作用减弱,没有显著的(p >0.05)最大响应或pD2值的变化。在两种温度下,L-NAME都能增强pe诱导的收缩,而在没有PVAT的主动脉段,这种作用更大。在两种温度下,PVAT主动脉段eNOS蛋白表达和一氧化氮(NO)的基础组织水平均较高。然而,PE仅在无PVAT的主动脉段显著增加组织NO水平。我们得出结论,PVAT诱导的冷却对pe诱导的收缩的抗收缩作用的丧失可能是由于PVAT损伤了主动脉段NO的生成。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 去求助
来源期刊
自引率
0.00%
发文量
0
期刊最新文献
Issue Information Autonomic and Autacoid Pharmacology: Goodbye and thank you Attenuation of the anti-contractile effect of cooling in the rat aorta by perivascular adipose tissue Retraction: Dopamine receptor immunohistochemistry in the rat choroid plexus. Issue Information
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
0
微信
客服QQ
Book学术公众号 扫码关注我们
反馈
×
意见反馈
请填写您的意见或建议
请填写您的手机或邮箱
已复制链接
已复制链接
快去分享给好友吧!
我知道了
×
扫码分享
扫码分享
Book学术官方微信
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术
文献互助 智能选刊 最新文献 互助须知 联系我们:info@booksci.cn
Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。
Copyright © 2023 Book学术 All rights reserved.
ghs 京公网安备 11010802042870号 京ICP备2023020795号-1