{"title":"Vibrational spectroscopic study of glutathione complexation in aqueous solutions","authors":"Michel Picquart, Lydie Grajcar, Marie Hélène Baron, Zohreh Abedinzadeh","doi":"10.1002/(SICI)1520-6343(1999)5:6<328::AID-BSPY2>3.0.CO;2-J","DOIUrl":null,"url":null,"abstract":"<p>A spectroscopic study of glutathione (GSH) and glutathione disulfide (GSSG) has been performed using Fourier-transformed infrared absorption and Raman scattering in order to pinpoint the sites of complexation of these two species with water and particularly with H<sub>2</sub>O<sub>2</sub>. Molecules of GSH and GSSG were studied in KBr pellets, and in aqueous solutions of H<sub>2</sub>O, D<sub>2</sub>O, and H<sub>2</sub>O with H<sub>2</sub>O<sub>2</sub> (1 mol L<sup>−1</sup>) to characterize the specific influence of the solvent molecules. A time-resolved Raman study was performed for GSH/H<sub>2</sub>O<sub>2</sub> in aqueous solution at 1 : 1 molar ratio in order to observe the formation of GSSG and to discuss the mechanism of this redox reaction. © 1999 John Wiley & Sons, Inc. Biospectroscopy 5: 328–337, 1999</p>","PeriodicalId":9037,"journal":{"name":"Biospectroscopy","volume":"5 6","pages":"328-337"},"PeriodicalIF":0.0000,"publicationDate":"1999-12-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1002/(SICI)1520-6343(1999)5:6<328::AID-BSPY2>3.0.CO;2-J","citationCount":"38","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Biospectroscopy","FirstCategoryId":"1085","ListUrlMain":"https://onlinelibrary.wiley.com/doi/10.1002/%28SICI%291520-6343%281999%295%3A6%3C328%3A%3AAID-BSPY2%3E3.0.CO%3B2-J","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 38
Abstract
A spectroscopic study of glutathione (GSH) and glutathione disulfide (GSSG) has been performed using Fourier-transformed infrared absorption and Raman scattering in order to pinpoint the sites of complexation of these two species with water and particularly with H2O2. Molecules of GSH and GSSG were studied in KBr pellets, and in aqueous solutions of H2O, D2O, and H2O with H2O2 (1 mol L−1) to characterize the specific influence of the solvent molecules. A time-resolved Raman study was performed for GSH/H2O2 in aqueous solution at 1 : 1 molar ratio in order to observe the formation of GSSG and to discuss the mechanism of this redox reaction. © 1999 John Wiley & Sons, Inc. Biospectroscopy 5: 328–337, 1999
水溶液中谷胱甘肽络合的振动光谱研究
利用傅里叶变换红外吸收和拉曼散射对谷胱甘肽(GSH)和谷胱甘肽二硫化(GSSG)进行了光谱研究，以确定这两种物质与水特别是与H2O2络合的位置。在KBr球团、H2O、D2O和H2O与H2O2 (1 mol L−1)的水溶液中研究了GSH和GSSG的分子，表征了溶剂分子的具体影响。为了观察GSH/H2O2在1:1摩尔比水溶液中氧化还原反应的形成，并探讨该氧化还原反应的机理，对GSH/H2O2进行了时间分辨拉曼研究。©1999 John Wiley &儿子,Inc。生物光谱学杂志，1999
本文章由计算机程序翻译，如有差异，请以英文原文为准。
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