Optimal N-ethyl-N-nitrosourea (ENU) doses for inbred mouse strains.

IF 1.5 4区生物学 Q3 Biochemistry, Genetics and Molecular Biology Genesis Pub Date : 2000-04-01

J S Weber, A Salinger, M J Justice

引用次数: 0

Abstract

ENU is a powerful germline mutagen in the mouse, providing the opportunity to analyze the functions of large numbers of genes in the mammalian genome. In many mutagenesis experiments, it would be beneficial to exploit the advantages of inbred mouse strains. To perform an effective ENU mutagenesis screen using inbred mice, a dosage regimen is required to determine the optimal dose of ENU for that inbred strain, a time-consuming preliminary process. We have carried out dosage regimens for mutagenizing doses of ENU in ten inbred strains of mouse: 129X1/SvJ, 129S6/SvEv, A/J, BALB/cJ, BTBR/N, C3He/J, C3HeB/FeJ, C57BL/6J, C57BR/cdJ, and CBA/CaJ, and determined an optimal dose for each strain, defined by length of sterile period and number of males to survive treatment. Three strains: A/J, BALB/cJ and C57BL/6J, are able to tolerate high doses, up to 300 mg/kg body weight, and are highly recommended for mutagenesis studies.

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近交系小鼠n -乙基-n -亚硝基脲(ENU)的最佳剂量。

ENU在小鼠中是一种强大的种系诱变剂，为分析哺乳动物基因组中大量基因的功能提供了机会。在许多诱变实验中，利用近交小鼠品系的优势是有益的。为了对近交系小鼠进行有效的ENU诱变筛选，需要确定一种剂量方案，以确定该近交系的ENU最佳剂量，这是一个耗时的初步过程。我们对10个自交系小鼠129X1/SvJ、129S6/SvEv、A/J、BALB/cJ、BTBR/N、C3He/J、C3HeB/FeJ、C57BL/6J、C57BR/cdJ和CBA/CaJ进行了ENU诱变剂量的给药方案，并根据不育期长度和存活雄性的数量确定了每个菌株的最佳剂量。三种菌株:A/J、BALB/cJ和C57BL/6J能够耐受高达300毫克/公斤体重的高剂量，被强烈推荐用于诱变研究。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Genesis DEVELOPMENTAL BIOLOGY-GENETICS & HEREDITY

CiteScore

3.90

自引率

0.00%

发文量

期刊介绍： As of January 2000, Developmental Genetics was renamed and relaunched as genesis: The Journal of Genetics and Development, with a new scope and Editorial Board. The journal focuses on work that addresses the genetics of development and the fundamental mechanisms of embryological processes in animals and plants. With increased awareness of the interplay between genetics and evolutionary change, particularly during developmental processes, we encourage submission of manuscripts from all ecological niches. The expanded numbers of genomes for which sequencing is being completed will facilitate genetic and genomic examination of developmental issues, even if the model system does not fit the “classical genetic” mold. Therefore, we encourage submission of manuscripts from all species. Other areas of particular interest include: 1) the roles of epigenetics, microRNAs and environment on developmental processes; 2) genome-wide studies; 3) novel imaging techniques for the study of gene expression and cellular function; 4) comparative genetics and genomics and 5) animal models of human genetic and developmental disorders. genesis presents reviews, full research articles, short research letters, and state-of-the-art technology reports that promote an understanding of the function of genes and the roles they play in complex developmental processes.