Capillary electrophoretic separation by double-strand polyaniline-coate capillaries of the advanced glycation endproducts formed from N-alpha-acetyl-L-lysine with reducing sugars.

Paula F G de Sa, Christina Robb, Elmo Resende, Patrick McCarthy, Sze C Yang, Phyllis R Brown, Joel A Dain
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Abstract

Capillary electrophoresis using a capillary coated with a double-strand coating of polyaniline:poly(methyacrylate-co-acrylic acid) (PAN:P[MA-AAI) was used to separate advanced glycation endproducts (AGEs) formed at 37 degrees C from model systems containing either glucose (Glc), fructose (Fru), or glyceraldehyde (GA) and N-alpha-acetyl-L-lysine (NALys). The presence of the P(MA-AA) as a second strand in the polymer allows the maintenance of the conductive state of the PAN at a wide pH range. Effects of buffer pH and coating concentration on the electroosmotic flow (EOF) were investigated. More AGE species can be detected for the GA/NALys mixtures using this coated capillary than upon an uncoated capillary. The coating procedure is simple and the stability of the coated capillary is good.

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用双链聚苯胺涂层毛细管对n -乙酰赖氨酸与还原糖形成的晚期糖基化终产物进行毛细管电泳分离。
毛细管电泳采用涂有聚苯胺双链涂层的毛细管:聚(甲基丙烯酸酯-共丙烯酸)(PAN:P[MA-AAI]),用于从含有葡萄糖(Glc)、果糖(Fru)或甘油醛(GA)和n - α -乙酰基-l -赖氨酸(NALys)的模型体系中分离在37℃形成的晚期糖基化终产物(AGEs)。P(MA-AA)作为第二链存在于聚合物中,使得PAN在较宽的pH范围内保持导电状态。研究了缓冲液pH和涂膜浓度对电渗透流动(EOF)的影响。在GA/NALys混合物中,使用这种涂覆毛细管比使用未涂覆毛细管可以检测到更多的AGE物种。涂覆过程简单,涂覆毛细管稳定性好。
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