Comparison of a thermo-associating matrix and a liquid polymer.

Nidhal Kahlaoui, Valessa Barbier, Marie-Alix Duval, Françoise Lefebvre, Jan Sudor, Rainer Siebert
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Abstract

Capillary electrophoresis is still widely used for DNA sequencing. The quality of the replaceable sieving matrix is a key area for massive sequencing with regard to speed and efficiency. The T25 polymer has been tested extensively and compared to poly(N,N-dimethylacrylamide) (PDMA). In terms of peak resolution, both polymers perform similarly. On the other hand, the run time is much shorter with the T25 polymer.

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热缔合基质与液体聚合物的比较。
毛细管电泳仍广泛用于DNA测序。可替换筛分基质的质量是大规模测序的速度和效率的关键领域。T25聚合物已经过广泛的测试,并与聚(N,N-二甲基丙烯酰胺)(PDMA)进行了比较。在峰值分辨率方面,两种聚合物的性能相似。另一方面,使用T25聚合物的运行时间要短得多。
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Chiral separation of FITC-labeled amino acids with gel electrochromatography using a polydimethylsiloxane microfluidic device. Analysis of sphingosine 1-phosphate by capillary electrophoresis coupled to laser-induced fluorescence detection: use of a transparent fused-silica capillary. Separation of homo- and heteroduplexes of DNA fragments with different melting temperature by capillary electrophoresis at one single temperature. Comparison of a thermo-associating matrix and a liquid polymer. Marja-Liisa Riekkola.
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