M Gourgues , P.-H Clergeot , C Veneault , J Cots , S Sibuet , A Brunet-Simon , C Levis , T Langin , M.-H Lebrun
{"title":"A new class of tetraspanins in fungi","authors":"M Gourgues , P.-H Clergeot , C Veneault , J Cots , S Sibuet , A Brunet-Simon , C Levis , T Langin , M.-H Lebrun","doi":"10.1016/S0006-291X(02)02355-0","DOIUrl":null,"url":null,"abstract":"<div><p>Tetraspanins are animal proteins involved in membrane complexes that are involved in cell adhesion, differentiation, and motility. The <em>PLS1</em><span> gene from rice blast fungus </span><em>Magnaporthe grisea</em> encodes a protein (Pls1p) structurally related to tetraspanins that is required for pathogenicity. In <span><em>Botrytis cinerea</em></span> public sequences, we identified an EST homologous to <em>PLS1</em><span>. Using degenerated oligonucleotides, we amplified sequences homologous to </span><em>PLS1</em> in fungi <span><em>Colletotrichum lindemuthianum</em></span> and <span><em>Neurospora crassa</em></span>. Analysis of <em>N. crassa</em> and <em>M. grisea</em><span><span> genome sequences revealed the presence of a single tetraspanin gene. Thus, fungi differ from animals, which contain between 20 and 37 paralogous tetraspanin genes. </span>Fungal proteins encoded by </span><em>BcPLS1</em>, <em>ClPLS1</em>, and <em>NcPLS1</em><span><span> display all the structural hallmarks of tetraspanins (predicted topology with four transmembrane domains, extra- and intracellular loops; conserved cysteine-based patterns in second extracellular loop). </span>Phylogenetic analysis suggests that these genes define a new family of orthologous genes encoding fungal-specific tetraspanins.</span></p></div>","PeriodicalId":8779,"journal":{"name":"Biochemical and biophysical research communications","volume":null,"pages":null},"PeriodicalIF":2.5000,"publicationDate":"2002-10-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0006-291X(02)02355-0","citationCount":"44","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Biochemical and biophysical research communications","FirstCategoryId":"99","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0006291X02023550","RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"BIOCHEMISTRY & MOLECULAR BIOLOGY","Score":null,"Total":0}
引用次数: 44
Abstract
Tetraspanins are animal proteins involved in membrane complexes that are involved in cell adhesion, differentiation, and motility. The PLS1 gene from rice blast fungus Magnaporthe grisea encodes a protein (Pls1p) structurally related to tetraspanins that is required for pathogenicity. In Botrytis cinerea public sequences, we identified an EST homologous to PLS1. Using degenerated oligonucleotides, we amplified sequences homologous to PLS1 in fungi Colletotrichum lindemuthianum and Neurospora crassa. Analysis of N. crassa and M. grisea genome sequences revealed the presence of a single tetraspanin gene. Thus, fungi differ from animals, which contain between 20 and 37 paralogous tetraspanin genes. Fungal proteins encoded by BcPLS1, ClPLS1, and NcPLS1 display all the structural hallmarks of tetraspanins (predicted topology with four transmembrane domains, extra- and intracellular loops; conserved cysteine-based patterns in second extracellular loop). Phylogenetic analysis suggests that these genes define a new family of orthologous genes encoding fungal-specific tetraspanins.
期刊介绍:
Biochemical and Biophysical Research Communications is the premier international journal devoted to the very rapid dissemination of timely and significant experimental results in diverse fields of biological research. The development of the "Breakthroughs and Views" section brings the minireview format to the journal, and issues often contain collections of special interest manuscripts. BBRC is published weekly (52 issues/year).Research Areas now include: Biochemistry; biophysics; cell biology; developmental biology; immunology
; molecular biology; neurobiology; plant biology and proteomics