Determination of colchicine content in Colchicum hierosolymitanum and Colchicum tunicatum under cultivation.

M Al-Fayyad, F Alali, Ahmad Alkofahi, Abdelmajeed Tell
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引用次数: 30

Abstract

Corms of Colchicum hierosolymitanum and Colchicum tunicatum were collected, identified and planted under field condition. We report here and for the first time the presence of colchicine in an appreciable amount in both species. The effect of different NPK fertilizer levels on colchicine content of the two Colchicum species at different growth stages were evaluated by HPLC. Results indicated that increasing NPK fertilizer levels significantly improve colchicine content in different plant parts and stages. The highest colchicine content observed in corms was at maturity stage 0.766 mg/g and 0.688 mg/g dry weight with C. hierosolimitanum and C. tunicatum, respectively.

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栽培秋水仙和秋水仙中秋水仙碱含量的测定。
对秋水仙(Colchicum hierosolymitanum)和秋水仙(Colchicum tunicatum)球茎进行了采集、鉴定和田间栽培。我们在这里首次报道了秋水仙碱在两个物种中都有相当数量的存在。采用高效液相色谱法测定了不同氮磷钾施量对两种秋水仙不同生育期秋水仙碱含量的影响。结果表明,增加氮磷钾施用量可显著提高秋水仙碱在植株各部位和各生育期的含量。秋水仙碱含量最高的是成熟期,干重分别为0.766 mg/g和0.688 mg/g。
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