Proteome analysis by mass spectrometry.

Annual review of biophysics and biomolecular structure Pub Date : 2003-01-01 Epub Date: 2003-01-28 DOI:10.1146/annurev.biophys.32.110601.141854

P Lee Ferguson, Richard D Smith

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引用次数: 135

Abstract

The coupling of high-performance mass spectrometry instrumentation with highly efficient chromatographic and electrophoretic separations has enabled rapid qualitative and quantitative analysis of thousands of proteins from minute samples of biological materials. Here, we review recent progress in the development and application of mass spectrometry-based techniques for the qualitative and quantitative analysis of global proteome samples derived from whole cells, tissues, or organisms. Techniques such as multidimensional peptide and protein separations coupled with mass spectrometry, accurate mass measurement of peptides from global proteome digests, and mass spectrometric characterization of intact proteins hold great promise for characterization of highly complex protein mixtures. Advances in chemical tagging and isotope labeling techniques have enabled quantitative analysis of proteomes, and highly specific isolation strategies have been developed aimed at selective analysis of posttranslationally modified proteins.

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质谱分析蛋白质组学。

高性能质谱仪器与高效色谱和电泳分离的耦合使得从生物材料的微小样品中快速定性和定量分析数千种蛋白质成为可能。在这里，我们回顾了质谱技术的发展和应用的最新进展，质谱技术用于对来自整个细胞、组织或生物体的蛋白质组样品进行定性和定量分析。诸如多维肽和蛋白质分离与质谱相结合的技术，从全局蛋白质组消化中精确测量肽的质量，以及完整蛋白质的质谱表征等技术，为表征高度复杂的蛋白质混合物提供了巨大的希望。化学标记和同位素标记技术的进步使蛋白质组的定量分析成为可能，并且开发了高度特异性的分离策略，旨在对翻译后修饰的蛋白质进行选择性分析。

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Annual review of biophysics and biomolecular structure

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