Effects of the tyrosine kinase inhibitor tyrphostin AG 556 on acute necrotising pancreatitis in rats.

Etem Alhan, Ramazan Cicek, Cengiz Erçin, Asim Orem, Birgül Vanizor, Akif Cinel
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Abstract

Objective: To investigate the effects of the tyrosine kinase inhibitor tyrphostin AG 556 on the course of acute necrotising pancreatitis in rats.

Design: Laboratory study.

Setting: Medical school, Turkey.

Animals: 72 Sprague Dawley rats, 12 in the sham operated (control) group and 20 in each of the three others.

Main outcome measures: Cardiorespiratory measurements, mortality, effect on the activities of various enzymes in serum and tissue of pancreas and lung, and the histological picture.

Results: The four study groups were sham + Ringer's lactate, acute necrotising pancreatitis with Ringer's lactate, tryphostin AG 556, and dimethylsulfoxide (DMSO). There were 12 animals in the first group and 20 in each of the other groups. The induction of pancreatitis increased mortality from 0/12 in the control to 6/20 (30%), 7/20 (35%), 8/20 (40%) in the three experimental groups, respectively. Heart rate, packed cell volume (PCV), serum activities of amylase and alanine aspartate transferase, tissue activity of myeloperoxidase (MPO) and malondialdehyde (MDA) in the pancreas and lung, serum concentrations of urea and calcium, volume of ascites, degree of pancreatic damage, blood pressure, and urine production did no differ between the pancreatitis groups.

Conclusions: Treatment with the tyrphostin kinase inhibitor did not improve the course of acute pancreatitis or reduce the extent of acinar cell injury and is therefore unlikely to be of benefit in patients with pancreatitis.

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酪氨酸激酶抑制剂tyrphostin AG 556对大鼠急性坏死性胰腺炎的影响。
目的:探讨酪氨酸激酶抑制剂tyrphostin AG 556对大鼠急性坏死性胰腺炎病程的影响。设计:实验室研究。背景:医学院,土耳其。实验动物:sd大鼠72只,假手术组12只,其他3组各20只。主要结果测量:心肺功能测量,死亡率,对血清和胰腺、肺组织中各种酶活性的影响,组织学图。结果:4个研究组分别为假手术+乳酸林格,急性坏死性胰腺炎伴乳酸林格,胰蛋白酶AG 556,二甲亚砜(DMSO)。第一组有12只动物,其他各组各有20只。胰腺炎的诱发使死亡率分别从对照组的0/12提高到6/20(30%)、7/20(35%)、8/20(40%)。心率、堆积细胞体积(PCV)、血清淀粉酶和丙氨酸天冬氨酸转移酶活性、胰腺和肺部髓过氧化物酶(MPO)和丙二醛(MDA)组织活性、血清尿素和钙浓度、腹水体积、胰腺损伤程度、血压和尿量在胰腺炎组之间没有差异。结论:用tyrphostin激酶抑制剂治疗并不能改善急性胰腺炎的病程或减少腺泡细胞损伤的程度,因此不太可能对胰腺炎患者有益。
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Influence of surgeon's volume on early outcome after total gastrectomy. Prospective evaluation of laparoscopic and open 360 degree fundoplication in mild and severe gastro-oesophageal reflux disease. Factors that affect the variability in heart rate during endoscopic retrograde cholangiopancreatography. Risk factors for severe postoperative hypocalcaemia after operations for primary hyperparathyroidism. Effects of the tyrosine kinase inhibitor tyrphostin AG 556 on acute necrotising pancreatitis in rats.
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