Stimulatory effect of prostaglandin F(2alpha) on Na-dependent phosphate transport in osteoblast-like cells.

IF 2.9 4区医学 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY Prostaglandins, leukotrienes, and essential fatty acids Pub Date : 2003-05-01 DOI:10.1016/s0952-3278(03)00021-8

I Sato, A Suzuki, A Kakita, Y Ono, Y Miura, M Itoh, Y Oiso

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引用次数: 7

Abstract

Prostaglandin F(2alpha) (PGF(2alpha)) has been reported to activate protein kinase C (PKC) through both phospholipase (PL) C and D, resulting in the proliferation of osteoblast-like cells. In addition, it has also been reported that Erk mitogen-activated protein kinase is also involved in the mechanism of PGF(2alpha)-induced proliferation of these cells. Recently, we have reported that several growth factors stimulate Na-dependent phosphate transport (Pi transport) activity of osteoblast-like cells, which has been recognized to play an important role in their mineralization. In the present study, we investigated the effect of PGF(2alpha) on Pi transport in MC3T3-E1 osteoblast-like cells. PGF(2alpha) stimulated Na-dependent Pi transport dose dependently in the range between 1nM and 10 micro M in MC3T3-E1 cells. The effect was time dependent up to 24h. Kinetic analysis revealed that PGF(2alpha) induces newly synthesized Pi transporter. Pretreatment with actinomycin D and cycloheximide suppressed PGF(2alpha)-induced enhancement of Pi transport. Combined effect of PMA and PGF(2alpha) was not additive in Pi transport. Calphostin C, a PKC inhibitor, dose-dependently suppressed Pi transport induced by PGF(2alpha). On the contrary, U0126, which inhibits an upstream kinase of Erk (MEK), did not affect PGF(2alpha)-induced enhancement of Pi transport. In conclusion, PGF(2alpha) stimulates Pi transport through activation of PKC in osteoblast-like cells.

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前列腺素F(2α)对na依赖性磷酸盐在成骨细胞样细胞中的转运的刺激作用。

据报道，前列腺素F(2alpha) (PGF(2alpha))通过磷脂酶(PL) C和D激活蛋白激酶C (PKC)，导致成骨细胞样细胞增殖。此外，也有报道称Erk丝裂原活化蛋白激酶也参与了PGF(2alpha)诱导这些细胞增殖的机制。最近，我们报道了几种生长因子刺激Na-dependent phosphate transport (Pi transport)活性，这在成骨细胞样细胞的矿化中起重要作用。在本研究中，我们研究了PGF(2alpha)对MC3T3-E1成骨样细胞中Pi转运的影响。在MC3T3-E1细胞中，PGF(2alpha)在1nM ~ 10 micro - M范围内以剂量依赖性刺激na依赖的Pi转运。效果与时间有关，最长可达24小时。动力学分析表明PGF(2alpha)诱导新合成的Pi转运体。放线菌素D和环己亚胺预处理可抑制PGF(2alpha)诱导的Pi转运增强。PMA和PGF(2alpha)的联合作用在Pi转运中不加和。Calphostin C, PKC抑制剂，剂量依赖性地抑制PGF(2alpha)诱导的Pi转运。相反，抑制Erk上游激酶(MEK)的U0126不影响PGF(2alpha)诱导的Pi转运增强。综上所述，PGF(2alpha)通过激活成骨细胞样细胞中的PKC刺激Pi转运。

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来源期刊

Prostaglandins, leukotrienes, and essential fatty acids 医学-内分泌学与代谢

CiteScore

6.40

自引率

6.70%

发文量

审稿时长

13.2 weeks

期刊介绍： The role of lipids, including essential fatty acids and their prostaglandin, leukotriene and other derivatives, is now evident in almost all areas of biomedical science. Cell membrane behaviour and cell signalling in all tissues are highly dependent on the lipid constituents of cells. Prostaglandins, Leukotrienes & Essential Fatty Acids aims to cover all aspects of the roles of lipids in cellular, organ and whole organism function, and places a particular emphasis on human studies. Papers concerning all medical specialties are published. Much of the material is particularly relevant to the development of novel treatments for disease.