Effects of essential fatty acids on mediators of mast cells in culture

IF 3.2 Prostaglandins, leukotrienes, and essential fatty acids Pub Date : 2003-05-01 DOI:10.1016/S0952-3278(03)00022-X

T. Gueck, A. Seidel, H. Fuhrmann

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Abstract

The objective of this study was to investigate the effects of α-linolenic acid (18:3n-3) and linoleic acid (18:2n-6) on the fatty acid composition and the activity and release of mast cell mediators in the canine mastocytoma cell line C2. Cells were cultured in Dulbecco's modified Eagle's medium mixed with 50% Ham's F12 (containing linoleic acid 0.14 μM). The basic medium (DEH) was supplemented with 0.14 μM α-linolenic acid. 14.0 μM α-linolenic acid (DEH-n-3) or 14.0 μM linoleic acid (DEH-n-6) was added. Eight days after culturing of C2 in DEH-n-3 we measured elevated levels of n-3 fatty acids up to 22:3. The tryptase activity and the stimulated PGE2 production and histamine release were reduced. In contrast, after culturing of C2 in DEH-n-6 we determined elevated levels of n-6 fatty acids up to 20:3, increased tryptase activity and stimulated histamine release. Thus 18:3n-3 has anti-inflammatory effects in cultured canine mastocytoma cells.

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必需脂肪酸对肥大细胞培养基的影响

本研究旨在探讨α-亚麻酸（18:3n-3）和亚油酸（18:2n-6）对犬肥大细胞瘤C2细胞株脂肪酸组成及肥大细胞介质活性和释放的影响。细胞在Dulbecco改良Eagle’s培养基中与50% Ham’s F12（含亚油酸0.14 μM）混合培养。基础培养基（DEH）中添加0.14 μM α-亚麻酸。添加14.0 μM α-亚麻酸（DEH-n-3）或14.0 μM亚油酸（DEH-n-6）。C2在DEH-n-3中培养8天后，我们测量到n-3脂肪酸水平升高至22:3。胰蛋白酶活性降低，受刺激的PGE2生成和组胺释放减少。相比之下，在DEH-n-6中培养C2后，我们发现n-6脂肪酸水平升高至20:3，胰蛋白酶活性增加，组胺释放刺激。由此可见，18:3n-3对培养的犬肥大细胞瘤细胞具有抗炎作用。

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