Involvement of dopamine D3 and neuropeptide Y Y5 receptors in diabetic gastroparetic rats without response to erythromycin.

Sheng wu hua xue yu sheng wu wu li xue bao Acta biochimica et biophysica Sinica Pub Date : 2003-09-01

Xin-Yu Qin, Zhi-Gang Wang, Jian Fei, Feng-Lin Liu, Da-Fu Cui, Shao-Liang Chen

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Abstract

Erythromycin may accelerate gastric emptying in animals and human probably as an motilin agonist, but its prokinetic effects show obvious individual disparity. This study was to find the mechanism of this phenomenon. Microarray analysis was used to screen genes that might be involved in the response of diabetic gastroparesis rats to erythromycin. It was found that erythromycin accelerated gastric emptying of diabetic rats with great individual disparity. Through microarray analysis we screened differential expression genes that might be involved in the effect of erythromycin. Among 10 genes screened out, dopamine D3 receptor (DRD3) and neuropeptide Y Y5 receptor (NPYY5) genes were submitted to RT-PCR quantification and showed consistent results with microarray. It can be concluded that erythromycin promote gastric emptying of gastroparetic rats; DRD3 and NPYY5 may be involved in prokinetic action of erythromycin; and targets other than motilin receptor of erythromycin might exist as prokinetics.

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多巴胺D3和神经肽yy5受体参与糖尿病胃轻瘫大鼠对红霉素无反应。

红霉素可能作为胃动素激动剂促进动物和人的胃排空，但其促排空作用存在明显的个体差异。本研究旨在找出这一现象的机理。采用微阵列分析筛选可能参与糖尿病胃轻瘫大鼠对红霉素反应的基因。发现红霉素对糖尿病大鼠胃排空有促进作用，个体差异较大。通过微阵列分析，我们筛选了可能参与红霉素作用的差异表达基因。筛选出的10个基因中，将多巴胺D3受体(DRD3)和神经肽YY5受体(NPYY5)基因进行RT-PCR定量，与微阵列结果一致。可见红霉素对胃轻瘫大鼠胃排空有促进作用;DRD3和NPYY5可能参与红霉素的促动力学作用;而红霉素除胃动素受体外的靶点也可能作为前动力学存在。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Sheng wu hua xue yu sheng wu wu li xue bao Acta biochimica et biophysica Sinica

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