[The immunopotentiation of human B lymphocyte stimulator C-terminal peptide].

Rui-Yang Tian, Wei Han, Ying Yu, Yue Chen, Gu-Song Yu, Sheng-Li Yang, Yi Gong
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Abstract

The cDNA of human B lymphocyte stimulator C-terminal peptide (C-BLyS) was amplified by nested PCR from cDNA library of human fetal brain. The expression plasmid pT7450-C-BLyS was constructed and transformed into E. coli BL21 CodonPlus (DE3) RIL which can recognize many rare codons. The C-BLyS protein was expressed as inclusion body in E. coli BL21 CodonPlus (DE3) RIL and the inclusion body of C-BLyS was denatured and then refolded by dialysis and purified by ion exchange chromatography. The refolded and purified C-BLyS can specifically bind with BCMA-Fc, a fusion protein of BLyS receptor and human IgG1 Fc. Furthermore, C-BLyS is proved to be an effective stimulator in mouse splenocytes proliferation in vitro and effective immunostimulant in vivo.

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人B淋巴细胞刺激物c端肽的免疫增强作用。
采用巢式PCR方法从人胎脑cDNA文库中扩增人B淋巴细胞刺激因子c -末端肽(C-BLyS) cDNA。构建表达质粒pT7450-C-BLyS,转化为大肠杆菌BL21 CodonPlus (DE3) RIL,可识别多种罕见密码子。C-BLyS蛋白在大肠杆菌BL21 CodonPlus (DE3) RIL中以包涵体的形式表达,通过透析使C-BLyS包涵体变性后再折叠,并用离子交换层析纯化。重组纯化的C-BLyS可特异性结合BLyS受体与人IgG1 Fc的融合蛋白BCMA-Fc。此外,C-BLyS在体外被证明是小鼠脾细胞增殖的有效刺激剂,在体内被证明是有效的免疫刺激剂。
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Analysis of gene expression in hepatitis B virus transfected cell line induced by interferon. [Cloning and expression of tumor necrosis factor (TNFalpha) cDNA from red seabream pagrus major]. [Effects of sodium selenite on telomerase activity and telomere length]. [Cloning, eukaryotic expression and function assay of recombinant leukemia inhibitory factor gene LIF]. [The immunopotentiation of human B lymphocyte stimulator C-terminal peptide].
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