{"title":"The hydrolysis of chitin by concentrated hydrochloric acid, and the preparation of low-molecular-weight substrate for lysozyme","authors":"J.A. Rupley","doi":"10.1016/0926-6526(64)90001-1","DOIUrl":null,"url":null,"abstract":"<div><p>The hydrolysis of chitin in solution in strong HCl was studied over a range of acid concentration and temperature. The data suggest that the rate of hydrolysis governed by the structure of the chitin. A simple procedure for the isolation of the oligosaccharides obtained from chitin was developed, for use in the preparation of low-molecular-weight substrates for lysozyme (<em>N</em>-acetylmuramide glycanohydrolase, EC 3.2.1.17). Experiments studying the lysozyme-catalyzed hydrolysis of these oligosaccharides showed a large effect of chain length upon the rate of hydrolysis, and no effect of ionic strength.</p></div>","PeriodicalId":100172,"journal":{"name":"Biochimica et Biophysica Acta (BBA) - Specialized Section on Mucoproteins and Mucopolysaccharides","volume":"83 3","pages":"Pages 245-255"},"PeriodicalIF":0.0000,"publicationDate":"1964-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0926-6526(64)90001-1","citationCount":"414","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Biochimica et Biophysica Acta (BBA) - Specialized Section on Mucoproteins and Mucopolysaccharides","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/0926652664900011","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 414
Abstract
The hydrolysis of chitin in solution in strong HCl was studied over a range of acid concentration and temperature. The data suggest that the rate of hydrolysis governed by the structure of the chitin. A simple procedure for the isolation of the oligosaccharides obtained from chitin was developed, for use in the preparation of low-molecular-weight substrates for lysozyme (N-acetylmuramide glycanohydrolase, EC 3.2.1.17). Experiments studying the lysozyme-catalyzed hydrolysis of these oligosaccharides showed a large effect of chain length upon the rate of hydrolysis, and no effect of ionic strength.
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