{"title":"Acid mucopolysaccharides in rat kidneys","authors":"D. Allalouf , A. Ber , N. Sharon","doi":"10.1016/0926-6526(64)90005-9","DOIUrl":null,"url":null,"abstract":"<div><p>An acid mucopolysaccharide preparation was isolated from rat kidneys. Its major amino sugar component was glucosamine, with only minor amounts of galactosamine. It contained sulphate, gave a positive carbazole reaction for uronic acids with a high carbazole: orcinol ratio, and possessed anticoagulant activity. The preparation could be further fractionated with the aid of cetyl pyridinium chloride, and by chromatography on columns of Ecteolal-cellulose and DEAE-Sephadex. From these studies it was concluded that the major component of the mucopolysaccharide preparation was a heparin-like substance, and that hyaluronic acid was present as a minor component. Since mucopolysaccharides containing galactosamine were found only in small quantities, it appears that rat kidneys do not contain significant amounts of chondroitin sulphates.</p></div>","PeriodicalId":100172,"journal":{"name":"Biochimica et Biophysica Acta (BBA) - Specialized Section on Mucoproteins and Mucopolysaccharides","volume":"83 3","pages":"Pages 278-287"},"PeriodicalIF":0.0000,"publicationDate":"1964-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0926-6526(64)90005-9","citationCount":"21","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Biochimica et Biophysica Acta (BBA) - Specialized Section on Mucoproteins and Mucopolysaccharides","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/0926652664900059","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 21
Abstract
An acid mucopolysaccharide preparation was isolated from rat kidneys. Its major amino sugar component was glucosamine, with only minor amounts of galactosamine. It contained sulphate, gave a positive carbazole reaction for uronic acids with a high carbazole: orcinol ratio, and possessed anticoagulant activity. The preparation could be further fractionated with the aid of cetyl pyridinium chloride, and by chromatography on columns of Ecteolal-cellulose and DEAE-Sephadex. From these studies it was concluded that the major component of the mucopolysaccharide preparation was a heparin-like substance, and that hyaluronic acid was present as a minor component. Since mucopolysaccharides containing galactosamine were found only in small quantities, it appears that rat kidneys do not contain significant amounts of chondroitin sulphates.
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