Metabolism in vitro of palmitic acid and linoleic acid in the heart and diaphragm of essential fatty acid-deficient rats

Olga Stein , Yecheskiel Stein
{"title":"Metabolism in vitro of palmitic acid and linoleic acid in the heart and diaphragm of essential fatty acid-deficient rats","authors":"Olga Stein ,&nbsp;Yecheskiel Stein","doi":"10.1016/0926-6542(64)90022-8","DOIUrl":null,"url":null,"abstract":"<div><p>Rats kept for 75–90 days on an essential fatty acid-deficient diet, showed a marked fall in linoleic acid content and a rise in the trienoic acid/tetreanoic acid ratio in heart as well as in diaphragm. Electron microscopic study of myocardium showed enlarged mitochondria with marked disorganization of cristae and swelling. Neutral lipid and phospholipid content of heart and diaphragm was not different from that of controls, supplemented with 4% soya-bean oil. Rat hearts were perfused and hemidiaphragms were incubated with a medium containing [1-<sup>14</sup>C]palmitic or [1-<sup>14</sup>C]linoleic acid. During 20 min of perfusion both fatty acids were oxidized to CO<sub>2</sub> to a similar extent. The fatty acid composition of the medium affected the mode and rate of incorporation of individual fatty acids in heart as well as diaphragm. In the presence of an equipmolar mixture of palmitic and linoleic acid, more linoleic acid was incorporated into phospholipids and more palmitic into neutral lipids, than when each fatty acid was the only substrate. In the essential fatty acid-deficient rats the specific activity of the phospholipids synthesized with [1-<sup>14</sup>C linoleic acid in heart as well as in diaphragm was higher than in controls. An increased turnover of certain fatty acids in phospholipids in the essential fatty acid-deficient rats is indicated and the mechanism discussed.</p></div>","PeriodicalId":100171,"journal":{"name":"Biochimica et Biophysica Acta (BBA) - Specialized Section on Lipids and Related Subjects","volume":"84 6","pages":"Pages 621-635"},"PeriodicalIF":0.0000,"publicationDate":"1964-12-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0926-6542(64)90022-8","citationCount":"26","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Biochimica et Biophysica Acta (BBA) - Specialized Section on Lipids and Related Subjects","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/0926654264900228","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 26

Abstract

Rats kept for 75–90 days on an essential fatty acid-deficient diet, showed a marked fall in linoleic acid content and a rise in the trienoic acid/tetreanoic acid ratio in heart as well as in diaphragm. Electron microscopic study of myocardium showed enlarged mitochondria with marked disorganization of cristae and swelling. Neutral lipid and phospholipid content of heart and diaphragm was not different from that of controls, supplemented with 4% soya-bean oil. Rat hearts were perfused and hemidiaphragms were incubated with a medium containing [1-14C]palmitic or [1-14C]linoleic acid. During 20 min of perfusion both fatty acids were oxidized to CO2 to a similar extent. The fatty acid composition of the medium affected the mode and rate of incorporation of individual fatty acids in heart as well as diaphragm. In the presence of an equipmolar mixture of palmitic and linoleic acid, more linoleic acid was incorporated into phospholipids and more palmitic into neutral lipids, than when each fatty acid was the only substrate. In the essential fatty acid-deficient rats the specific activity of the phospholipids synthesized with [1-14C linoleic acid in heart as well as in diaphragm was higher than in controls. An increased turnover of certain fatty acids in phospholipids in the essential fatty acid-deficient rats is indicated and the mechanism discussed.

查看原文
分享 分享
微信好友 朋友圈 QQ好友 复制链接
本刊更多论文
棕榈酸和亚油酸在必需脂肪酸缺乏大鼠心脏和膈肌的体外代谢
饲喂缺乏必需脂肪酸饮食75-90天的大鼠,心脏和膈肌的亚油酸含量明显下降,三烯酸/四烯酸比值明显上升。电镜观察心肌线粒体增大,嵴明显紊乱,肿胀。添加4%大豆油后,心脏和膈肌中性脂和磷脂含量与对照组无显著差异。大鼠心脏灌注,半隔膜用含有[1-14C]棕榈酸或[1-14C]亚油酸的培养基孵育。在灌注20 min时,两种脂肪酸被氧化为CO2的程度相似。培养基的脂肪酸组成影响个别脂肪酸在心脏和隔膜的掺入方式和速率。在棕榈酸和亚油酸的混合物中,与每种脂肪酸作为唯一底物相比,更多的亚油酸被掺入磷脂中,更多的棕榈酸被掺入中性脂中。必需脂肪酸缺乏大鼠心脏和膈肌中[1-14C亚油酸合成的磷脂比活性均高于对照组。必需脂肪酸缺乏大鼠的磷脂中某些脂肪酸的周转增加,并讨论了其机制。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 去求助
来源期刊
自引率
0.00%
发文量
0
期刊最新文献
Author index Subject index Errata Errata Metabolism in vitro of palmitic acid and linoleic acid in the heart and diaphragm of essential fatty acid-deficient rats
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
0
微信
客服QQ
Book学术公众号 扫码关注我们
反馈
×
意见反馈
请填写您的意见或建议
请填写您的手机或邮箱
已复制链接
已复制链接
快去分享给好友吧!
我知道了
×
扫码分享
扫码分享
Book学术官方微信
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术
文献互助 智能选刊 最新文献 互助须知 联系我们:info@booksci.cn
Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。
Copyright © 2023 Book学术 All rights reserved.
ghs 京公网安备 11010802042870号 京ICP备2023020795号-1