Myo-inositol phosphates in a phosphoinositide complex from kidney

Abstract

• 1.

  1. Chemical studies on the “phosphatido-peptide fraction” from kidney have shown that this material more properly should be called a phosphoinositide complex.

• 2.

  2. Glycerophosphate, inositol mono-, di- and triphosphate were separated from alkaline hydrolysates by means of paper chromatography.

• 3.

  3. Six fractions were obtained by column chromatography from the deacylated phosphoinositide complex and identified as inositol monophosphate, glycerophosphate, inositol diphosphate, glycerylphosphoryl inositol, glycerylphosphoryl inositol phosphate, and glycerylphosphoryl inositol diphosphate.

• 4.

  4. These phosphates, presumably as the inositides, are believed to be responsible for the hgh metabolic activity of this fraction as measured by radiophosphorus.