{"title":"Fractionation and characterization of the low-density lipoproteins of hen's egg yolk","authors":"W.G. Martin, J. Augustyniak, W.H. Cook","doi":"10.1016/0926-6542(64)90029-0","DOIUrl":null,"url":null,"abstract":"<div><p>The LDF of hen's egg yolk prepared by a modified method was found to be soluble in water. In this low-density solvent it was possible to separate LDF by flotation into fractions (LDF<sub>1</sub> and LDF<sub>2</sub>) owing in part to the maximum effect of the difference in their partial specific volumes. About one-fifth of LDF was estimated to consist of LDF<sub>1</sub>, the remainder being LDF<sub>2</sub>. Both fractions were polydisperse with molecular weights ranging from 0.5 · 10<sup>6</sup> to 34 · 10<sup>6</sup> (mol. wt. 10.3 · 10<sup>6</sup>) for LDF<sub>1</sub> and 0.5 · 10<sup>6</sup> to 14 · 10<sup>6</sup> (mol. wt. 3.3 · 10<sup>6</sup>) for LDF<sub>2</sub>. Since LDF<sub>1</sub> contained 86.8% and LDF<sub>2</sub> 83.2% lipid, the mean size of their protein moieties was about 1.4 · 10<sup>6</sup>, respectively.</p></div>","PeriodicalId":100171,"journal":{"name":"Biochimica et Biophysica Acta (BBA) - Specialized Section on Lipids and Related Subjects","volume":"84 6","pages":"Pages 714-720"},"PeriodicalIF":0.0000,"publicationDate":"1964-12-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0926-6542(64)90029-0","citationCount":"94","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Biochimica et Biophysica Acta (BBA) - Specialized Section on Lipids and Related Subjects","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/0926654264900290","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 94
Abstract
The LDF of hen's egg yolk prepared by a modified method was found to be soluble in water. In this low-density solvent it was possible to separate LDF by flotation into fractions (LDF1 and LDF2) owing in part to the maximum effect of the difference in their partial specific volumes. About one-fifth of LDF was estimated to consist of LDF1, the remainder being LDF2. Both fractions were polydisperse with molecular weights ranging from 0.5 · 106 to 34 · 106 (mol. wt. 10.3 · 106) for LDF1 and 0.5 · 106 to 14 · 106 (mol. wt. 3.3 · 106) for LDF2. Since LDF1 contained 86.8% and LDF2 83.2% lipid, the mean size of their protein moieties was about 1.4 · 106, respectively.
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