Relationship between endothelin 1 and nitric oxide system in the corpus luteum regression

IF 3.2 Prostaglandins, leukotrienes, and essential fatty acids Pub Date : 2003-11-01 DOI:10.1016/j.plefa.2003.07.002

T. Tognetti, A. Estevez, C.G. Luchetti, V. Sander, A.M. Franchi, A.B. Motta

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Abstract

The present study was designed to investigate the relationship between the nitric oxide (NO) system and endothelin 1 (ET-1) in the mechanism of corpus luteum (CL) development and consequently regression in rats. We first evaluated basal ET-1 levels in ovarian tissue from rats with different stages of CL development. An increased ovarian ET-1 content was found during CL regression. In a dose-department response, ET-1 decreased progesterone (P4) and increased prostaglandin (PG) PGF2α production. By means of a competitive nitric oxide synthase (NOS) inhibitor: L-nitro arginine methyl ester (L-NAME) and a slow NO releasing: diethyl-aminetriamine (DETA-NONOate), we demonstrated that NO system could be the intermediary in the ET-1 diminishing P4 production. The Western blot analysis revealed an increase on iNOS while eNOS protein expression was diminished. We also found a diminution of total NOS activity after ET-1 treatment. These data suggest the existence of a functional relationship between ET-1 and NOS isoforms leading the regulation of CL functionally.

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黄体退化过程中内皮素1与一氧化氮系统的关系

本研究旨在探讨一氧化氮（NO）系统与内皮素-1 （ET-1）在大鼠黄体（CL）发育及退化机制中的关系。我们首先评估了不同CL发展阶段大鼠卵巢组织中ET-1的基础水平。卵巢ET-1含量在CL回归中升高。在剂量系反应中，ET-1降低孕酮（P4）并增加前列腺素（PG） PGF2α的产生。通过竞争性一氧化氮合酶（NOS）抑制剂：l-硝基精氨酸甲酯（L-NAME）和缓慢释放NO的：二乙基胺三胺（delta - nonoate），我们证明NO系统可能是ET-1减少P4产生的中介。Western blot分析显示，iNOS表达增加，eNOS蛋白表达减少。我们还发现ET-1治疗后总NOS活性降低。这些数据表明ET-1和NOS同工异构体之间存在功能关系，导致CL的功能调节。

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