Proteomic technology and its biomedical applications.

Andy T Y Lau, Qing-Yu He, Jen-Fu Chiu
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Abstract

Proteomics has its origins in two-dimensional gel electrophoresis (2-DE), a technique developed more than twenty years ago. 2-DE has a high-resolution capacity, and was initially used primarily for separating and characterizing proteins in complex mixtures. 2-DE remains an important tool for protein identification, but is now normally coupled with mass spectrometry (MS), a technique which has advanced considerably in recent years. The recent completion of human genome project has produced a large DNA database which can be utilized through bioinformatics, and the next challenge for scientists is to uncover the entire proteome of a particular organism. The integration of genomic and proteomic data will help to elucidate the functions of proteins in the pathogenesis of diseases and the ageing process, and could lead to the discovery of novel drug target proteins and biomarkers of diseases. This review describes recent advances in proteomic technology and discusses the potential applications of proteomics in biomedical research.

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蛋白质组学技术及其生物医学应用。
蛋白质组学起源于二十多年前发展起来的二维凝胶电泳(2-DE)技术。2-DE具有高分辨率的能力,最初主要用于分离和表征复杂混合物中的蛋白质。2-DE仍然是蛋白质鉴定的重要工具,但现在通常与质谱(MS)相结合,这是一种近年来取得长足进步的技术。最近人类基因组计划的完成已经产生了一个可以通过生物信息学来利用的大型DNA数据库,科学家的下一个挑战是揭示特定生物体的整个蛋白质组。基因组学和蛋白质组学数据的整合将有助于阐明蛋白质在疾病发病机制和衰老过程中的功能,并可能导致发现新的药物靶蛋白和疾病的生物标志物。本文综述了蛋白质组学技术的最新进展,并讨论了蛋白质组学在生物医学研究中的潜在应用。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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