{"title":"[Effect of ligustrazine on the expression of Bcl-2 protein and apoptosis in rabbit articular chondrocytes in monolayer culture].","authors":"Sheng Xiao, Kang-hua Li, Hong-Bin Lu","doi":"","DOIUrl":null,"url":null,"abstract":"<p><strong>Objective: </strong>To determine the effect of ligustrazine on the expression of Bcl-2 protein and apoptosis in rabbit articular chondrocytes in monolayer culture.</p><p><strong>Methods: </strong>We separated chondrocytes from the rabbit articular cartilage and cultured them in vitro. We divided them into 2 groups. One group was added the injective ligustrazine hydrochloride(LH), and the other was added the same quantity of culture medium as the comparison. The expression of Bcl-2 in every group was examined with immunohistocytochemistry technique, and the apoptosis was observed with TUNEL.</p><p><strong>Results: </strong>The two groups both expressed Bcl-2 protein, but the expression in the LH group was significantly higher than that in the comparison group (P < 0.01). The two groups both had apoptosis, the rate of which in the LH group was significantly lower than that in the comparison group (P < 0.01).</p><p><strong>Conclusion: </strong>Ligustrazine may repair the cartilaginous lesion.</p>","PeriodicalId":13115,"journal":{"name":"Hunan yi ke da xue xue bao = Hunan yike daxue xuebao = Bulletin of Hunan Medical University","volume":"28 3","pages":"224-6"},"PeriodicalIF":0.0000,"publicationDate":"2003-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Hunan yi ke da xue xue bao = Hunan yike daxue xuebao = Bulletin of Hunan Medical University","FirstCategoryId":"1085","ListUrlMain":"","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0
Abstract
Objective: To determine the effect of ligustrazine on the expression of Bcl-2 protein and apoptosis in rabbit articular chondrocytes in monolayer culture.
Methods: We separated chondrocytes from the rabbit articular cartilage and cultured them in vitro. We divided them into 2 groups. One group was added the injective ligustrazine hydrochloride(LH), and the other was added the same quantity of culture medium as the comparison. The expression of Bcl-2 in every group was examined with immunohistocytochemistry technique, and the apoptosis was observed with TUNEL.
Results: The two groups both expressed Bcl-2 protein, but the expression in the LH group was significantly higher than that in the comparison group (P < 0.01). The two groups both had apoptosis, the rate of which in the LH group was significantly lower than that in the comparison group (P < 0.01).
Conclusion: Ligustrazine may repair the cartilaginous lesion.
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