[Particle enhanced immunoturbidimetic assay of high sensitive C-reactive protein].

Min Hu, Yan-wu Li, Yong-hong Hu
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Abstract

Objective: To evaluate the particle enhanced immunoturbidimetic assay of high sensitive C-reactive protein by the automated chemistry analyzer (Hitachi 7170A).

Methods: The detection limit of quantification, measurement range, precision, interference and reference interval were assessed.

Results: The measurement range of the method was from 0.125 to 12.5 mg/L. The detection limitation was 0.115 mg/L. The internal and external accuracy were 0.89% and 0.90% respectively. The reference range of the assay was from 0.20 mg/L to 2.60 mg/L.

Conclusion: The particle enhanced immunoturbidimetic assay is sensitive and stable, and can be used in clinical diagnosis.

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[颗粒增强免疫比浊法测定高敏感c反应蛋白]。
目的:评价全自动化学分析仪(日立7170A)颗粒增强免疫比浊法检测高敏c反应蛋白的方法。方法:对其定量检出限、测量范围、精密度、干扰度和参考区间进行评价。结果:方法测定范围为0.125 ~ 12.5 mg/L。检出限为0.115 mg/L。内、外准确度分别为0.89%、0.90%。该方法的参考范围为0.20 ~ 2.60 mg/L。结论:颗粒增强免疫比浊法灵敏、稳定,可用于临床诊断。
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