[Impact of non-target gene mutations and reduced permeability of outer membrane on quinolone resistance and multiple antibiotic resistance in Escherichia coli].

Hunan yi ke da xue xue bao = Hunan yike daxue xuebao = Bulletin of Hunan Medical University Pub Date : 2003-06-01

Rui-hua Zhao, Ming-xing Shu, Shu-zhen Chen

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Abstract

Objective: To investigate the impact of non-target gene mutations and reduced permeability of the outer membrane on quinolone resistance and multiple antibiotic resistance (mar) in Escherichia coli.

Methods: Several high-level quinolone resistant isolates whose strains were accompanied by concurrent resistant to several antibiotics were selected. The PCR products of marOR region were sequenced to detect the possible gene changes and then the strains' outer membrane proteins (Omps) were extracted to analyze on the constitutive profiles.

Results: We found two site mutations in R410 isolate: 1,870(T-->C, Val-->Ala) and 1,879(A-->C, terminator-->Ser). The OmpF deficiency was found in all 3 resistant strains.

Conclusion: The increased expression of MarA induced by gene mutations in marOR and the constitutive profile changes of the Omps (OmpF deficient strains) may play some role in the quinolone resistance and multiple antibiotic resistance of Escherichia coli.

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[非靶基因突变和外膜通透性降低对大肠杆菌喹诺酮类药物耐药和多种抗生素耐药的影响]。

目的:探讨非靶基因突变和外膜通透性降低对大肠杆菌喹诺酮类药物耐药和多种抗生素耐药(mar)的影响。方法:选择对几种抗生素同时耐药的高水平喹诺酮类耐药菌株。对marOR区的PCR产物进行测序，检测可能的基因变化，然后提取菌株的外膜蛋白(Omps)进行组成谱分析。结果:我们在R410分离物中发现两个位点突变:1870 (T- >C, Val- >Ala)和1879 (A- >C，终止子->Ser)。所有3株耐药菌株均存在OmpF缺陷。结论:基因突变引起的marOR中MarA表达升高及Omps (OmpF缺陷菌株)的组成谱变化可能与大肠埃希菌的喹诺酮类药物耐药和多种抗生素耐药有关。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Hunan yi ke da xue xue bao = Hunan yike daxue xuebao = Bulletin of Hunan Medical University

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