PCR-based detection of a cystic fibrosis epidemic strain of Pseudomonas Aeruginosa.

Molecular diagnosis : a journal devoted to the understanding of human disease through the clinical application of molecular biology Pub Date : 2003-01-01 DOI:10.1007/BF03260038

Stavroula Panagea, Craig Winstanley, Yasmin N Parsons, Martin J Walshaw, Martin J Ledson, C Anthony Hart

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引用次数: 19

Abstract

Background: The Liverpool epidemic strain (LES) of Pseudomonas aeruginosa is widespread among patients with cystic fibrosis (CF) in specialist centers around Liverpool and elsewhere in the UK. This study evaluates a new diagnostic PCR assay based on a unique DNA sequence (PS21) of LES, for its identification of colonies directly from sputum.

Methods: One hundred and fifty-eight sputum samples from 92 patients were cultured and P. aeruginosa isolates were typed by PS21 PCR and pulsed-field gel electrophoresis (PFGE). Subsequently, PS21 PCR was performed directly on sputum and the results were compared with culture, PFGE, and PS21 PCR typing.

Results: Eighty patients were colonized with P. aeruginosa, 63 by LES (79%). There was 100% concordance between PS21 PCR on colonies and PFGE typing. The sensitivity and specificity of PS21 PCR directly on sputum was 98.2% and 93.6%, respectively.

Conclusions: This study shows that PS21 PCR can be used for simple and rapid screening of LES colonization in CF patients.

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一株铜绿假单胞菌囊性纤维化流行株的pcr检测。

背景:利物浦流行菌株(LES)铜绿假单胞菌在利物浦周围的专科中心和英国其他地方的囊性纤维化(CF)患者中广泛存在。本研究评估了一种新的基于LES独特DNA序列(PS21)的诊断PCR方法，用于直接从痰中鉴定菌落。方法:对92例患者158份痰标本进行培养，采用PS21 PCR和脉冲场凝胶电泳(PFGE)对铜绿假单胞菌进行分型。随后，直接对痰液进行PS21 PCR，并将结果与培养、PFGE和PS21 PCR分型进行比较。结果:80例患者有铜绿假单胞菌定植，63例为LES定植(79%)。菌落PS21 PCR与PFGE分型的一致性为100%。PS21 PCR直接检测痰液的敏感性为98.2%，特异性为93.6%。结论:本研究表明PS21 PCR可用于CF患者LES定殖的简单快速筛选。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Molecular diagnosis : a journal devoted to the understanding of human disease through the clinical application of molecular biology

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